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MetabolomicsIntegrating two chromatographies in a co-fractionation mass spectrometry approach improved protein-metabolite interactions (PMIs) mapping capabilities and revealed novel protein-ligand pairings in Escherichia coli.
The protein-metabolite interactome remains understudied. We previously developed a co-fractionation mass-spectrometry-based approach called PROMIS to address this gap. PROMIS relies on size separation chromatography of protein-metabolite complexes from lysates, followed by proteomics and metabolomic...
2025-04-25 | MTBLS9098 | MetaboLights
MetabolomicsMetabolomic Sequencing of Cell-Free Supernatant from Bacillus strain 541

The 4-6 minute fraction from the HPLC fractionation of the bacterial culture supernatant of porcine-derived probiotic Bacillus strain 541.

2025-09-08 | MTBLS12956 | MetaboLights
TranscriptomicsMembrane-polysome associated fractionation of HES2 embryonic stem cells
The membrane fraction and the cytosolic fraction of HES2 cells were collected and subjected to microarray. The experiment was performed in triplicate Keywords: membrane-polysome fractionation of RNA Three consecutive passages of HES2 cells were grown and subject to membrane-polysome fractionation....
ORGANISM(S): Homo sapiens 
2010-04-07 | E-GEOD-14037 | biostudies-arrayexpress
TranscriptomicsBiochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA
Biochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA. Nuclei from three biological replicates were isolated by detergent lysis, fractionated, then three chromatin and three soluble RNA samples were converted to cDNA using Illumina TruSeq stranded pro...
ORGANISM(S): Homo sapiens 
2015-03-04 | E-GEOD-66478 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of human HeLa cell lines at different cell fractionation stages
We investigated RNAs involved in the maintenance of spatial chromatin organization during interphase. Various RNA fractions from HeLa cells were prepared, tested in in vivo experiments and sequenced in order to identify and characterize the RNA fraction presenting the higher activity.
ORGANISM(S): Homo sapiens 
2012-03-03 | E-MTAB-582 | biostudies-arrayexpress
ProteomicsIEF fractionation OVCAR-5 cell line
Analysis of the ovarian cancer cell line OVCAR-5. A standard trypsin digest was carried out on the OVCAR-5 cell lysates which were then analysed in the un-fractionated and fractionated forms. Fractionation was completed using a peptide IEF separation method. All samples were analysed by nano-LC-ESI-...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD003547 | Pride
Ief fractionation lc-ms/ms ovarian cancer cell line 
ProteomicsComparison of fractionation proteomics for local SWATH library building
For data-independent acquisition by means of sequential window acquisition of all theoretical fragment ion spectra (SWATH), a reference library of data-dependent acquisition (DDA) runs is typically used to correlate the quantitative data from the fragment ion spectra with peptide identifications. Th...
ORGANISM(S): Homo sapiens (Human) 
2017-07-04 | PXD006190 | Pride
Library building Comparative study Swath-ms Fractionation proteomics Hesc 
GenomicsCell fractionation reveals mis-annotation of human microRNAs
Cell fractionation reveals mis-annotation of human microRNAs
PRJNA762239 | ENA
TranscriptomicsGenomewide demarcation of RNA PolII transcription units by physical fractionation of chromatin- ORF enrichment
Epigenetic modifications of chromatin serve an important role in regulating the expression and accessibility of genomic DNA. We report here a genomewide approach for fractionating yeast chromatin into two functionally distinct parts, one containing RNA polymerase II transcribed sequences, and the ot...
ORGANISM(S): Saccharomyces cerevisiae 
2006-08-30 | E-GEOD-5649 | biostudies-arrayexpress
ProteomicsIPG Strip-Based Peptide Fractionation for Shotgun Proteomics
This deep proteome dataset of human HEK293 cells was generated using IPG Strip-Based fractionation.
ORGANISM(S): Homo sapiens (Human) 
2015-07-08 | PXD002389 | Pride
Hek293 Ipg fractionation 
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