Sort   by:  
 Page size 
MetabolomicsMultiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment
Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal t...
2021-09-27 | MTBLS1531 | MetaboLights
MetabolomicsThe Bardet-Biedl protein Bbs1 controls photoreceptor outer segment protein and lipid composition

Summary

Primary cilia are key sensory organelles whose dysfunction leads to ciliopathy disorders such as Bardet-Biedl syndrome (BBS). Retinal degeneration is common in ciliopathies, since the outer segments (OSs) of photoreceptors are highly specialized primary cilia. BBS...

2022-02-18 | MTBLS4013 | MetaboLights
MetabolomicsTear lipidomics profiling reveals inflammatory dry eye disease-associated lipid metabolic remodeling at the ocular surface
This study aimed to investigate whether inflammatory dry eye disease (DED) is accompanied by abnormal lipid metabolic remodeling at the ocular surface. Tear samples were collected from patients with DED and matched healthy controls. Lipidomics profiling was performed using LC–MS/MS with a standardiz...
2026-04-03 | MTBLS14218 | MetaboLights
MetabolomicsN-glycosylation Profiling of Colorectal Cancer Cell Lines Reveals Association of Fucosylation with Differentiation and Caudal Type Homebox 1 (CDX1)/Villin mRNA Expression
Various cancers such as colorectal cancer (CRC) are associated with alterations in protein glycosylation. CRC cell lines are frequently used to study these (glyco)biological changes and their mechanisms. However, differences between CRC cell lines with regard to their glycosylation have hitherto bee...
2017-02-03 | MTBLS227 | MetaboLights
MetabolomicsThe Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments

Apicomplexan parasite growth and replication relies on nutrient acquisition from host cells, in which intracellular multiplication occurs, yet the mechanisms that underlie the nutrient salvage remain elusive. Numerous ultrastructural studies have documented a plasma membrane invagination with a d...

2023-04-19 | MTBLS7006 | MetaboLights
MetabolomicsThe novel protein circPETH-147aa regulates metabolic reprogramming in hepatocellular carcinoma cells to remodel immunosuppressive microenvironment
Metabolic reprogramming fuels cancer cell metastasis and remodels the immunosuppressive tumor microenvironment (TME). We report here that a circRNA, circPETH, packaged by extracellular vesicles (EVs) from tumor-associated macrophages (TAMs) to hepatocellular carcinoma (HCC) cells, facilitates glycol...
2024-12-09 | MTBLS11254 | MetaboLights
MetabolomicsThe Poly (ADP-Ribose) Polymerase inhibitor Veliparib and Radiation Cause Significant Cell Line Dependent Metabolic Changes in Breast Cancer Cells
Breast tumors are characterized into different subtypes based on their surface marker expression, which affects their prognosis and treatment. For example, triple negative breast cancer cells (ER-/PR-/Her2-) show reduced susceptibility towards radiotherapy and chemotherapeutic agents. Poly (ADP-ribo...
2016-11-07 | MTBLS337 | MetaboLights
ProteomicsStratifying the Cell Surface Cysteinome using Two-step Enrichment Proteomics
Cell surface cysteines represent an attractive class of residues for chemoproteomic studies due to their accessibility towards drugs and key roles they play in the structure and function of most human proteins. The redox sensitivity of cysteines also makes cell surface cysteines potential markers fo...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD042403 | Pride
Oxidation states T cell activation Cysteines Chemoproteomics Cell surface 
ProteomicssBioSITe enables sensitive identification of the cell surface proteome through direct enrichment of biotinylated peptides
Background: Cell surface proteins perform critical functions related to immune response, signal transduction, cell-cell interactions, and cell migration. Expression of specific cell surface proteins can determine cell-type identity, and can be altered in diseases including infections, cancer and gen...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD044519 | Pride
Biosite Label-free quantitation Biotinylation Surfaceome 
ProteomicsA magnetic bead-based workflow for sensitive and streamlined cell surface proteomics
Cell-surface proteins represent an important class of molecules for therapeutic targeting and defining cellular phenotypes. However, their enrichment and detection via mass spectrometry-based proteomics remains challenging due to low abundance, posttranslational modifications, hydrophobic regions, a...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-01-21 | PXD043678 | Pride
Lc-ms  mass spectrometry  surfaceomics  cell surface capture  n-glycopeptide enrichment  plasma membrane n-glycoprotein 
Sort   by:  
 Page size