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MetabolomicsPhenotypes and Multi—omics Reveal Changes and Molecular Mechanism of Suspension Adaptation of HEK293 Cells Structural Remodelling, Metabolic Reconstruction and Stress Resistance

Human embryonic kidney 293 (HEK293) cells have been successfully adapted from adherent to suspension culture and have been widely applied in both scientific research and the pharmaceutical industry. However, the alterations in cells during the adaptation have not been well described, which raise ...

2025-12-16 | MTBLS13517 | MetaboLights
MetabolomicsMetabolomic Responses of Arabidopsis Suspension Cells to Bicarbonate under Light and Dark Conditions
Global CO2 level presently recorded at 400 ppm is expected to reach 550 ppm in 2050, an increment likely to impact plant growth and productivity. Using targeted LC-MS and GC-MS platforms we quantified 229 and 29 metabolites, respectively in a time-course study to reveal short-term responses to diffe...
2017-04-25 | MTBLS311 | MetaboLights
TranscriptomicsGene expression over the course of xylem tracheary element formation in cell suspension cultures of Arabidopsis thaliana
Transcriptomic analysis of gene expression during the differentiation of cell suspension cultures into tracheary elements using the biological system published by Pesquet et al., Current Biology (2010): tracheary element differentiation was triggered by externally supplying hormone-free habituated c...
ORGANISM(S): Arabidopsis thaliana 
2015-09-18 | E-GEOD-73146 | biostudies-arrayexpress
ProteomicsCharacterization of extracellular vesicles from cell suspension 2 cultures of Coffea arabica L.
A protocol was developed for the isolation and characterization of extracellular vesicles (EVs) from Coffea arabica cell suspension cultures (CSC). The isolation method involved differential ultracentrifugation of the CSC filtrate, yielding two fractions: 100,000 g for 36 minutes (100k g) and 125,00...
ORGANISM(S): Coffea arabica (Arabian coffee) 
2026-02-16 | PXD071909 | Pride
Cell suspension cultures  coffea arabica  extracellular vesicles (evs)  morpho-34 metric traits  proteomics  unconventional protein secretion 
ProteomicsProteomic analysis of plasmodesmata from Populus cell suspension cultures
In this project, we have investigated the proteome of the plasmodesmata (PD) enriched membrane fraction from poplar cell suspension cultures and quantitatively compared its protein composition with other membrane fractions. We build on the previously published literature on the PD proteome by using ...
ORGANISM(S): Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) 
2018-12-31 | PXD010692 | Pride
Mass spectrometry Plasmodesmata Callose Populus Spectral counting Callose synthase 
TranscriptomicsGene expression response of Populus tremuloides cell suspension cultures to salicyl alcohol feeding
Aspen cell cultures were fed 5 mM salicyl alcohol at the early exponential phase (5 days after subculture). Cells were harvested from fed (SL) or unfed (C) cells after 48 hours. Gene expression changes were analyzed using a custom 7K aspen EST array. RNA was extracted from 3 independent control (C)...
ORGANISM(S): Populus tremuloides 
2010-05-15 | E-GEOD-18360 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Medicago truncatula cell suspension cultures treated with invertase and/or S.meliloti lipopolysaccharide
This experiment studies the effect of different compounds on gene expression in M. truncatula cell cultures.
ORGANISM(S): Medicago truncatula 
2006-12-31 | E-MEXP-924 | biostudies-arrayexpress
TranscriptomicsIso-Seq analysis of cell suspension culture and plantlets of Hypericum perforatum
Iso-Seq (PacBio) sequencing was performed to generate a reference library of H. perforatum. We generated genome-wide transcriptome data from in vitro cell suspensions and shoot cultures of H. perforatum.
ORGANISM(S): Hypericum perforatum 
2022-12-31 | E-MTAB-11423 | biostudies-arrayexpress
MetabolomicsSingle-cell and bulk level metabolomic study of the glycolysis inhibition by 2-deoxy-D-glucose in Saccharomyces cerevisiae
Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In a study of the metabolic response of a clonal Saccharomyces cerevisiae population challenged by the glycolytic inhibitor 2-deoxy-D-glucose (2-DG), this variability is shown ...
2013-05-22 | MTBLS29 | MetaboLights
ProteomicsHeat stress triggers protein secretion into the extracellular matrix of sorghum cell suspension cultures
Plants reprogram gene expression as an adaptive response to survive high temperatures. While the identity and functions of intracellular heat stress-responsive proteins have been extensively studied, the heat response of proteins secreted to the extracellular matrix is unknown. Here, we used Sorghum...
ORGANISM(S): Sorghum bicolor 
2020-10-29 | PXD021536 | Pride
Sorghum Itraq Heat stress 
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