The mini-hairpin shaped nascent peptide that blocks translation termination was found by comprehensive proteomics approach for small ORFs overexpression.
The peptides obtained from RNA fraction (with TriPURE isolation reagent). Two strains (WT and delta-pth2), and three technical replicates for each strain.
Proteome analysis of a conditional GroE expression strain and its variants. Proteome data for the dnaKJ-deleted strain and its variant were also included.