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Precursor ion intensity quantification and intensity-based absolute quantity estimation (IBAQ) was used to on the mixture of yeast cell lysates in order to estimate the absolute concentrations of the proteins.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-06 | PXD012803 | Pride
In recent years, more attention in systems biology has been given to the concept of protein constraints and the cell’s necessity to allocate its proteome between important processes. From this point of view, attempts to elucidate cellular maximum capacity of growth as a function of protein availabil...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-08-05 | PXD018361 | Pride
We have studied Schizosaccharomyces pombe, Saccharomyces cerevisiae, Scheffersomyces (Pichia) stipites, and Kluyveromyces marxianus yeast using the modified IBAQ label-free method with the spiked universal protein standard (UPS2). Each species had been prepared in 3 culturing replicates, and the sim...
ORGANISM(S): Schizosaccharomyces pombe 927 Scheffersomyces stipitis Kluyveromyces marxianus Saccharomyces cerevisiae (Baker's yeast) 
2021-11-29 | PXD026313 | Pride
Tandem mass tag (TMT)-based relative quantification was used to study yeast metabolism under the stepwise reduction the nitrogen content in the growth medium.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-13 | PXD014962 | Pride
The absolute protein amounts for the selected yeast ribosomal proteins (RPs) were determined using the standard peptides and TMT labelling. The range of the standard tryptic peptides were selected and synthesized based on the preliminary data-dependent experiments. The synthetic peptides were mixed ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-13 | PXD015025 | Pride
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