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ProteomicsAurora A phosphorylates PKM2 at T45.
Flag-PKM2 was expressed in H1299 cells. Then the H1299 cells were treated with DMSO or 100nM MLN8237. Then, cell lysate was incubated with anti-Flag M2-agarose. Elutes were separated with 10% SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. Visualized bands were excised and subjected to ...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD036575 | Pride
Aurora a  pkm2 
Proteomics293T Flag-Aurora-A Co-IP LC-MSMS
To explore the potential targets of Aurora-A in glycolytic regulation
ORGANISM(S): Homo sapiens (Human) 
2019-11-21 | PXD016110 | Pride
Human Lc-msms 293t Flag-aurora-a co-ip 
ProteomicsIdentification of the quantitative phosphoproteome in mitotic HeLa cells with or without G6PD inhibitors.
HeLa cells were synchronized with a double thymidine block procedure. Briefly, the exponentially growing HeLa cells were maintained with 2 mM thymidine for 18 h, followed by a release of 9 h in fresh medium, and then cells were re-cultured in 2 mM thymidine for additional 15 h. After a release of 7....
ORGANISM(S): Homo sapiens (Human) 
2023-05-18 | PXD033825 | Pride
G6pd Mitosis Phosphoproteome 
ProteomicsIdentification of the potential regulators of G6PD activity in different cell cycle
Flag-G6PD was expressed in HeLa cells. Then the HeLa cells were synchronized with a double thymidine block procedure. Briefly, the exponentially growing HeLa cells were maintained with 2 mM thymidine for 18 h, followed by a release of 9 h in fresh medium, and then cells were re-cultured in 2 mM thym...
ORGANISM(S): Homo sapiens (Human) 
2023-05-18 | PXD033829 | Pride
G6pd Cell cycle 
UnknownMitotic Assembly of Respiratory Supercomplexes Ensures Accurate Cell Division and Fuels Tumor Growth
Not available
S-SCDT-10_1038-S44319-026-00937-8 | biostudies-other
UnknownCDK1-Mediated Phosphorylation of LDHA Fuels Mitosis through LDHB-Dependent Lactate Oxidation
Not available
S-SCDT-10_1038-S44319-025-00573-8 | biostudies-other
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