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MetabolomicsWhat CHO is made of: Variations in the biomass composition of Chinese hamster ovary cell lines

Background: Cell line specific, genome-scale metabolic models enable rigorous and systematic in silico investigation of cellular metabolism. Such models have recently become available for Chinese hamster ovary (CHO) too. However, a key ingredient, namely an experimentally validat...

2020-07-13 | MTBLS1210 | MetaboLights
TranscriptomicsGene expression profiles during the establishment of CHK-Q (Chinese hamster kidney-derived cell line) and derivative cells
CHK-Q cell line was established from primary culture of Chinese hamster kidney tissues by repeated passages. DNA microarray analysis for chasing the variation in gene expression profile was performed for CHK cells during the culture process leading to immortalization and adaptation to serum-free and...
ORGANISM(S): Cricetulus griseus 
2022-03-16 | E-MTAB-11298 | biostudies-arrayexpress
ProteomicsChinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 4
Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular m...
ORGANISM(S): Cricetulus Griseus (ncbitaxon:10029) 
2018-01-25 | MSV000081976 | MassIVE
ProteomicsChinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 3
Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular m...
ORGANISM(S): Cricetulus Griseus (ncbitaxon:10029) 
2018-02-14 | MSV000082065 | MassIVE
ProteomicsChinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 1
Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular m...
ORGANISM(S): Cricetulus Griseus (ncbitaxon:10029) 
2018-01-25 | MSV000081974 | MassIVE
ProteomicsChinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 2
Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular m...
ORGANISM(S): Cricetulus Griseus (ncbitaxon:10029) 
2018-01-25 | MSV000081975 | MassIVE
ProteomicsTargeted Quantitation of Host Cell Proteins by Capillary LC/IMS/MS/MS in Combination with Rapid Digestion on Immobilized Trypsin Column Under Native Conditions
Host cell protein (HCP) impurities are considered a critical quality attribute of biopharmaceuticals because of their potential to compromise safety and efficacy, and LC/MS-based analytical methods have been developed to identify and quantify individual proteins instead of employing enzyme-linked im...
ORGANISM(S): Cricetulus Griseus 
2025-05-02 | PXD053135 | JPOST Repository
TranscriptomicsCHO smallRNA sequencing for microRNA annotation
MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from CHO cells provide new tools for the discovery of novel miRNAs in this important production system. The current release of the miRNA re...
ORGANISM(S): Cricetulus griseus 
2015-03-02 | E-GEOD-59838 | biostudies-arrayexpress
ProteomicsEnrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis
Chinese hamster ovary (CHO) cells have been widely employed for production of recombinant proteins (RP) for research and therapeutic purposes. Indeed, this expression system was used for production of most of approved antibodies (84%) in 2015-2018 period. The success of this cell line for RP product...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-08-27 | PXD019778 | Pride
Organelles Subcellular fractionation Subcellular proteomics Chinese hamster ovary cells 
TranscriptomicsCHO cells with enhanced and abolished gamma-secretase activity
γ-secretase is an intra-membrane-cleaving aspartyl protease implicated in the processing of a wide range of type I membrane proteins including the Notch receptor and the amyloid-β precursor protein (APP). It thus regulates a diverse array of cellular and biological processes including the differen...
ORGANISM(S): Cricetulus griseus 
2010-05-15 | E-GEOD-16379 | biostudies-arrayexpress
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