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We have repurposed standard phosphoproteomics workflow based on Fe3+IMAC for enrichment ofmannose-6-phosphate modified glycopeptides. This worklfow was used to profile lysosomal acid hydrolases in HeLa and CHO cell lines. We have combined this approach with CRISPR/Cas9 KO of acid phosphatases 2 and ...
ORGANISM(S): Homo sapiens (Human) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2018-12-18 | PXD010333 | Pride
Imd, Basket and Relish (the Drosophila JNK and NF-kB homolog, respectively) genes were knock-downed individually by RNAi, and we compared the gene expression patterns in response to LPS with those of control samples treated with luciferase RNAi.
ORGANISM(S): Drosophila melanogaster 
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