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ProteomicsQuantitative Proteomics Analysis of the Cervicovaginal Mucus from 4 Individual Donors
The goal of this study was to identify amylases that might be present in the vaginal fluid from four individual donors coming either from the microbiome or expressed by the human donors in these fluids. We collected cervicovaginal mucus from 4 donors, characterized the species composition of vaginal...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-05-05 | MSV000085378 | MassIVE
ProteomicsCELA1 and AAT deficient murine model proteome
Chymotrypsin-like elastase 1 (CELA1) is a serine protease that is neutralized by alpha-1 antitrypsin (AAT) and prevents emphysema in a murine antisense oligonucleotide model of AAT-deficient emphysema. We tested the role of CELA1 in emphysema development in this genetic model of AAT-deficiency follo...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-02-20 | MSV000091330 | MassIVE
ProteomicsSuccinylome-controlled antibiotic resistance in MRSA - Succinylome
A sucC and a sucD mutant from the Nebraska Transposon Mutant Library (NTML, Strain NE569 and NE1770) were found to be susceptible to cefoxitin and oxacillin when other mutations in TCA cycle genes did not affect their resistance to these antibiotics. The SucC strain (Strain NE569) accumulated Succin...
ORGANISM(S): Staphylococcus Aureus (ncbitaxon:1280) 
2021-03-02 | MSV000086971 | MassIVE
ProteomicsSuccinylome-controlled antibiotic resistance in MRSA - Global proteome
A sucC and a sucD mutant from the Nebraska Transposon Mutant Library (NTML, Strain NE569 and NE1770) were found to be susceptible to cefoxitin and oxacillin when other mutations in TCA cycle genes did not affect their resistance to these antibiotics. The SucC strain (Strain NE569) accumulated Succin...
ORGANISM(S): Staphylococcus Aureus (ncbitaxon:1280) 
2021-03-02 | MSV000086976 | MassIVE
ProteomicsTracheal aspirate proteome from hospitalized patients infected with SARS-CoV-2
Tracheal aspirate was collected from intubated patients with SARS-CoV-2 infections or for reasons not related to COVID-19 (controls). The proteins were extracted using the MPLEx method. Proteins were digested with trypsin and analyzed by LC-MS/MS on a Thermo Q Exactive HF-X. Data was searched with M...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-06-06 | MSV000089614 | MassIVE
ProteomicsEvaluating linear ion trap incorporation for MS3-based multiplexed single-cell proteomics
The project aims to evaluate the use of a linear ion trap to increase the sensitivity of multiplexed single-cell proteomics. Using diluted mouse peptide samples from C10 and Raw, we compared the proteome coverages, data missingness, and quantification performance between linear ion trap and Orbitrap...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-08-22 | MSV000090178 | MassIVE
ProteomicsRat Bronchoalveolar Lavage Proteome Changes Following E-cigarette Aerosol Exposures
Label free quantitative proteomics of bronchoalveolar lavage (BAL) fluid collected from rats exposed to e-cigarette aerosols (humidified air control, PG/VG, PG/VG + Nicotine, or PG/VG + Nicotine + Vanillin)
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) 
2023-01-06 | MSV000091015 | MassIVE
ProteomicsIon mobility fractionation enhanced mass spectrometry acquisition method for single-cell proteomics
Development and evaluation of the TIFF (Transferring Identification based on FAIMS Fractionation) method by benchmarking with standard methods. Single HeLa cells, LPS-treated RAW cells, and non-depleted human lung dissociated single cells were analyzed as applications of the TIFF method for single c...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2020-08-10 | MSV000085937 | MassIVE
ProteomicsHigh-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip
Development and evaluation of nested nanowell (N2) chip in single-cell proteomics. The newly designed chip helps to improve the sensitivity and robustness of the tandem mass tag labeling approach by sizing down wells.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2021-02-04 | MSV000086809 | MassIVE
MetabolomicsGNPS - Comprehensive Characterization of Extracellular Vesicles by Environmental (Neff) and Clinical (T4) Strains of Acanthamoeba castellanii
Proteomics, lipidomics, and metabolomics analysis of extracellular vesicles secreted by an environmental strain and a clinical strain of Acanthamoeba castellanii.
ORGANISM(S): Acanthamoeba Castellanii (ncbitaxon:5755) 
2024-04-05 | MSV000094477 | GNPS
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