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ProteomicsBiochemical and structural characterization of PPEP-3 from Geobacillus thermodenitrificans
The members of the group of Pro-Pro endopeptidases (PPEPs) are secreted bacterial endoproteases that display a unique preference for hydrolyzing their substrates between two proline residues. The active site cleft of PPEPs accommodates the six substrate residues P3 to P3’, and the interactions betwe...
ORGANISM(S): Geobacillus thermodenitrificans 
2025-11-11 | PXD061585 | Pride
Geobacillus thermodenitrificans Macromolecular x-ray crystallography Zinc metallopeptidase Mass spectrometry Substrate specificity Pro-pro endopeptidase-3 
ProteomicsCharacterization of the Clostridioides difficile 630Δerm putative Pro-Pro endopeptidase CD1597
Clostridioides difficile is the leading cause of antibiotic-associated infections worldwide. Within the host, C. difficile can transition from a sessile to a motile state by secretion of PPEP-1, which releases the cells from the intestinal epithelium by cleaving adhesion proteins. PPEP-1 belongs to ...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2024-05-27 | PXD052347 | Pride
Tmtpro Proteomics Sporulation Clostridioides difficile Proteases Pro-pro endopeptidases 
ProteomicsAntigen-specific IgG Glycosylation Profiles in Hamsters and Macaques Following COVID-19 Vaccination
Vaccination triggers the production of antigen-specific antibodies, including IgG. IgG molecules are glycosylated at the Fc region, and these glycan modifications markedly influence Fc receptor binding and downstream immune functions. Notably, infections with enveloped viruses such as SARS-CoV-2 can...
ORGANISM(S): Mesocricetus auratus (Golden hamster) Macaca mulatta (Rhesus macaque) 
2026-03-11 | PXD068257 | Pride
Glycosylation Antibody Sars-cov-2 Vaccination Covid-19 
ProteomicsProteolytic activity of surface exposed HtrA determines its expression level and is needed to survive acidic conditions in Clostridioides difficile
To survive in the host, pathogenic bacteria need to be able to react to the unfavourable conditions that they encounter, like low pH, elevated temperatures, antimicrobial peptides and many more. These conditions may lead to unfolding of envelope proteins and this may be lethal. One of the mechanisms...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2025-11-11 | PXD050242 | Pride
Acid stress Clostridioides difficile Pdz domain Surface proteins Dimethyl labeling Htra Gene regulation Protease Lc-ms/ms 
ProteomicsA revised model for the Type A glycan biosynthetic pathway in Clostridioides difficile strain 630Δerm based on quantitative proteomics of cd0241-cd0244 mutant strains
The bacterial flagellum is involved in a variety of processes including motility, adherence and immunomodulation. In the Clostridioides difficile strain 630Δerm, the main filamentous component, FliC, is post-translationally modified with an O-linked Type A glycan structure. This modification is esse...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2024-01-26 | PXD045152 | Pride
Biosynthesis Proteomics Mass spectrometry Enzyme Glycation Flagella 
ProteomicsNon-prime- and Prime-side Profiling of Pro-Pro Endopeptidase Specificity Using Synthetic Combinatorial Peptide Libraries and Mass Spectrometry
A group of bacterial proteases, the Pro-Pro endopeptidases (PPEPs), possess the unique ability to hydrolyze proline-proline bonds in proteins. Since a protease’s function is largely determined by its substrate specificity, methods that can extensively characterize substrate specificity are valuable ...
ORGANISM(S): Anoxybacillus tepidamans Paenibacillus alvei Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2025-11-11 | PXD050236 | Pride
Clostridioides difficile Paenibacillus alvei Ppep Protease Specificity Pro-pro endopeptidase Anoxybacillus tepidamans Lc-ms/ms 
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