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ProteomicsIn-situ Click Reaction Coupled with Quantitative Proteomics for Identification of Protein Targets of Catechol Estrogens
Catechol estrogens (CEs) are metabolic electrophiles that actively undergo covalent interaction with cellular proteins, influencing molecular function. There is no feasible method to identify their binders in a living system. Herein, we developed a click chemistry-based approach using ethinylestradi...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-02-28 | PXD009657 | Pride
Post translational modification Metabolites Proteomics Electrophiles Dimethyl labeling Lc/ms Catechol estrogens Oxidative stress Click chemistry Redox modification 
ProteomicsClick-linking: a cell-compatible protein crosslinking method based on click chemistry
We developed a novel approach to in situ crosslinking mass spectrometry (XL-MS) where the reaction is split into two sequential and orthogonal coupling events. The method involves pre-stabilization of the proteome using a fixation protocol, followed by a two-step process that begins with extensive l...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-01-28 | PXD059118 | Pride
In situ Crosslinking Proteome-wide Protein-protein interactions Interactome 
GenomicsRna_seq analysis of muscle in click and EDC treated mouse
Rna_seq analysis of muscle in click and EDC treated mouse
PRJNA1054043 | ENA
TranscriptomicsProfiling of chromatin deposition bias in RPE-1 cells using Double-click-seq and SCAR-seq
We used double-click-seq and SCAR-seq methods to profile chromatin deposition bias in RPE-1 cells. Untreated cells and treatments promoting G4 formation (hydroxyurea, NMM) and their combination were profiled for characterizing chromatin protein redistribution asymmetry during DNA replication. This i...
ORGANISM(S): Homo sapiens 
2025-08-31 | E-MTAB-13346 | biostudies-arrayexpress
TranscriptomicsProfiling of chromatin deposition bias in RPE-1 cells using Double-click-seq
We used double-click-seq method to profile chromatin deposition bias in RPE-1 cells. Untreated wild-type, p53 knock-out and several treatments (hydroxyurea, ATR inhibitor, curaxin) and their combinations were profiled for characterizing assymetry during DNA replication.
ORGANISM(S): Homo sapiens 
2021-10-04 | E-MTAB-8624 | biostudies-arrayexpress
TranscriptomicsRna_seq analysis of muscle in click and EDC treated mouse
First, two groups of mice were subjected to hindlimb ischemic modeling. Then, peptide hydrogels synthesized by EDC reaction and click reaction were injected into the ischemic limbs of each group of mice, respectively. Several days later, RNA-seq analysis was performed on the ischemic limb muscles of...
ORGANISM(S): Mus musculus 
2023-12-23 | GSE250423 | GEO
UnknownA Template-Mediated Click-Click Reaction: PNA-DNA, PNA-PNA (or Peptide) Ligation, and Single Nucleotide Discrimination.
Not available
S-EPMC3597112 | biostudies-literature
UnknownPost-Functionalization of Organometallic Complexes via Click-Reaction.
Not available
S-EPMC9614606 | biostudies-literature
UnknownConformation of G-quadruplex Controlled by Click Reaction.
Not available
S-EPMC7570587 | biostudies-literature
UnknownFabrication of Hypericin Imprinted Polymer Nanospheres via Thiol-Yne Click Reaction.
Not available
S-EPMC6418589 | biostudies-literature
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