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MetabolomicsQuantitative metabolic profiles of tomato flesh and seeds during fruit development: complementary analysis with ANN and PCA (NMR data)
Global approach to characterize changes in metabolic profiles in two interdependent tissues Seed and Flesh from the same tomato fruits during tomato fruit development. Tomato, an essential crop in terms of economic importance and nutritional quality, is also used as a model species for all fleshy fr...
2013-09-24 | MTBLS56 | MetaboLights
ProteomicsTMTPro Complementary Ion Quantification Increase Plexing and Sensitivity for Accurate Multiplexed Proteomics at the MS2 level
The ability of the TMTPro isobaric labeling reagents to form complementary ions for accurate multiplexed proteomics at the MS2 level was investigated. Human and yeast peptides were labeled in distinct ratios to analyze the effect of interference on the quantification accuracy. A method, TMTProC, was...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2021-05-11 | PXD021661 | Pride
Complementary ion quantification Interference-free quantification Multiplexing Tmtpro 
TranscriptomicsRibonuclease L and Metal-Ion-Independent Endoribonuclease Cleavage Sites in Host and Viral RNAs
We used 2', 3'-cyclic phosphate cDNA synthesis and Illumina sequencing to identify and quantify metal-ion-independent endoribonuclease cleavage sites in host and viral RNAs. To make cDNA libraries, we exploited the 2’, 3’-cyclic phosphate at the end of RNA fragments produced by RNase L and other met...
ORGANISM(S): Homo sapiens 
2014-02-07 | E-GEOD-52489 | biostudies-arrayexpress
UnknownTMTpro Complementary Ion Quantification Increases Plexing and Sensitivity for Accurate Multiplexed Proteomics at the MS2 Level.
Not available
S-EPMC8330405 | biostudies-literature
TranscriptomicsHighly sensitive amplicon-based transcript quantification by semi-conductor sequencing
We have adapted a commercial assay that employs mRNA quantification based on the frequency of PCR amplicons determined by next-generation to a high-throughput semi-conductor sequencing platform (Ion-Torrent Proton). We show parallel amplification of pathway derived transcript sets/genes in 12 refere...
ORGANISM(S): Homo sapiens 
2014-07-15 | E-GEOD-58737 | biostudies-arrayexpress
ProteomicsSensitive and accurate proteome profiling of embryogenesis using Real-Time Search and TMTproC quantification
Multiplexed proteomics has become a powerful tool for investigating biological systems. Using balancer-peptide conjugates (e.g., TMTproC complementary ions) in the MS2 spectra for quantification circumvents the ratio distortion problem inherent in multiplexed proteomics. However, TMTproC quantificat...
ORGANISM(S): Ciona intestinalis x Ciona robusta Drosophila melanogaster (Fruit fly) Xenopus laevis (African clawed frog) 
2025-01-17 | PXD055647 | Pride
Ciona Tmtpro Xenopus Drosophila Multiplexing Ascend 
UnknownA Collision-Induced Dissociation Cleavable Isobaric Tag for Peptide Fragment Ion-Based Quantification in Proteomics.
Not available
S-EPMC7476077 | biostudies-literature
TranscriptomicsInnate lymphoid cell development requires TOX-dependent generation of a common ILC progenitor
Subtypes of innate lymphoid cells (ILC), defined by effector function and transcription factor expression, have recently been identified. In the adult, ILC derive from common lymphoid progenitors in bone marrow, although transcriptional regulation of the developmental pathways involved remains po...
ORGANISM(S): Mus musculus 
2015-04-27 | E-GEOD-65850 | biostudies-arrayexpress
ProteomicsComplementary peptides (cPEPs) modulate protein translation
MicroRNAs (miRNAs) are small regulatory RNAs that control plant and animal development by regulating post-transcriptional gene expression. Primary transcripts of miRNAs contain small ORFs that can be translated into small peptides (miPEPs). MiPEPs specifically enhance the expression of their cognate...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-01-09 | PXD017404 | Pride
Affinity purification Microrna Post-transcriptional regulation Complementary peptides 
ProteomicsQuantification of ErbB network proteins in three cell types using complementary approaches identifies cell general and cell type-specific signaling proteins
Relating protein concentration to cell-type-specific responses is one of the remaining challenges for obtaining a quantitative systems level understanding of mammalian signaling. Here we used mass-spectrometry (MS)- and antibody-based quantitative proteomic approaches to measure protein abundances f...
ORGANISM(S): Homo sapiens (Human) 
2013-12-18 | PXD000623 | Pride
Human Hek293 Primary keratinocyltes Quantification 
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