Covalent capture

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DNA unmethylome profiling by covalent capture of CpG sites

Here we present an approach for the mapping of unmethylated DNA. Our technique is based on DNA methyltransferase-directed transfer of activated groups (mTAG) and covalent biotin tagging of unmodified CpG sites followed by affinity enrichment and interogation on tiling microarrays or next generation ...

ORGANISM(S): Homo sapiens

2013-08-27 | E-GEOD-48994 | biostudies-arrayexpress

Identification of Bona Fide Targets of AMPylating Fic-enzymes by Substrate Mediated Covalent Capture

Many pathogenic bacteria utilize posttranslational modifications to manipulate central components of the host cell functions. Often, the released enzymes belong to the large Fic-family, causing target modification with nucleotide monophosphates. Due to the lack of a generic method for the identifica...

ORGANISM(S): Homo sapiens (Human)

2020-07-09 | PXD015599 | Pride