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ProteomicsBiocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cells
Fluorescent probes enable the visualization of dynamic cellular processes with high precision, particularly when coupled with super-resolution imaging techniques that surpass the diffraction limit. Traditional methods include fluorescent protein fusion (e.g., GFP) or organic fluorophores linked to l...
ORGANISM(S): Sus Scrofa (ncbitaxon:9823) 
2025-11-18 | MSV000099944 | MassIVE
ProteomicsDevelopment of covalent probes to capture Legionella pneumophila effector enzymes
Upon infection of host cells Legionella pneumophila releases a multitude of effector enzymes into the hosts cytoplasm that hijack a plethora of cellular activities, including the hosts ubiquitination pathways. Effectors belonging to the SidE-family are involved in non-canonical serine phosphoribosyl...
ORGANISM(S): Homo sapiens (Human) Legionella pneumophila subsp. pneumophila Lp02 
2025-05-06 | PXD049797 | Pride
Legionella pneumophila Activity-based probe Ubiquitylation Adp-ribosylation Bacterial infection 
ProteomicsOlaparib based photo-affinity probes for PARP-1 detection in living cells
The poly-ADP-ribose polymerase (PARP) is a protein from the family of ADP-ribosyltransferases that catalyzes poly adenosine diphosphate ribose (ADPR) formation in order to attract the DNA repair machinery to DNA damage sites. Inhibition of PARP activity by olaparib can cause cell death which is of c...
ORGANISM(S): Homo sapiens (Human) 
2020-04-29 | PXD018661 | Pride
Olaparib  parp-1  bioorthogonal chemistry  chemical proteomics  photoaffinity probes 
ProteomicsLysine-targeting, covalent inhibitors of bromodomain BD1 of BET proteins in live cells and animals
We report herein a set of activity-based probes (ABPs; P3-P7) based on various lysine-reactive covalent warheads capable of global profiling of ligandable lysine within BRDs in live cells and animals. Chemoproteomic experiments with P7 by utilizing 2-ethynylbenzaldehyde (EBA) identified 18 endogenou...
ORGANISM(S): Homo Sapiens Mus Musculus 
PXD058513 |
ProteomicsSulfamate acetamides as self-immolative electrophiles for covalent ligand- directed release chemistry
Electrophiles for covalent inhibitors that are suitable for in vivo administration are rare. While acrylamides are prevalent in FDA approved covalent drugs, chloroacetamides are considered too reactive for such purposes. We report sulfamate-based electrophiles that maintain chloroacetamide-like geom...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD038301 | Pride
Human Chemical probes Isodtb 
UnknownSelective nucleic acid capture with shielded covalent probes.
Not available
S-EPMC3703666 | biostudies-literature
ProteomicsSulfamate acetamides as self-immolative electrophiles for covalent ligand- directed release chemistry - Pull Down data
Electrophiles for covalent inhibitors that are suitable for in vivo administration are rare. While acrylamides are prevalent in FDA approved covalent drugs, chloroacetamides are considered too reactive for such purposes. We report sulfamate-based electrophiles that maintain chloroacetamide-like geom...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD038375 | Pride
Human Pull down Chemical probes 
UnknownAffinity-based covalent sialyltransferase probes enabled by ligand-directed chemistry.
Not available
S-EPMC11749336 | biostudies-literature
UnknownCovalent docking of large libraries for the discovery of chemical probes.
Not available
S-EPMC4232467 | biostudies-literature
ProteomicsLight-activatable, 2,5-disubstituted tetrazoles for the proteome-wide profiling of aspartates and glutamates in living bacteria
Covalent inhibitors have recently seen a resurgence of interest in drug development. Nevertheless, compounds, that do not rely on an enzymatic activity, have almost exclusively been developed to target cysteines. Expanding the scope to other amino acids would be largely facilitated by the ability to...
ORGANISM(S): Escherichia coli Staphylococcus aureus 
2020-04-14 | PXD016659 | Pride
Covalent inhibitors Antibiotics Chemoproteomics Isodtb-abpp 
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