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ProteomicsDegrasyn exhibits antibiotic activity against multi-resistant Staphylococcus aureus by modifying several essential cysteines
In this dataset, we investigate the targets of degrasyn in the methicillin-sensitive S. aureus strain NCTC 8325. This includes data on enrichment studies and competition studies with conventional ABPP using a degrasyn-derived probe, competition studies with residue-specific proteomics using the isoD...
ORGANISM(S): Staphylococcus aureus 
2020-04-14 | PXD016413 | Pride
Chemoproteomics Antibioitcs S. aureus Isodtb-abpp 
ProteomicsisoDTB-ABPP Cysteine Chemoproteomic Profiling for bigelovin
isoDTB-ABPP Cysteine Chemoproteomic Profiling was used to detect the targets of bigelovin in NLRP3 inflammasome activation. LPS and ATP induced bone marrow derived macrophages were adopted for the experiment in three biological replicates. Cell was treated with DMSO and bigelovin respectively. The c...
ORGANISM(S): Mus Musculus 
PXD053000 |
ProteomicsTargeted Protein Localization by Covalent 14-3-3 Recruitment
chemoproteomic profiling of covalent ligands that target 14-3-3
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-08-28 | PXD054696 | Pride
14-3-3 
ProteomicsIsocyanides inhibit bacterial pathogents by covalent targeting of essential metabolic enzymes
Isonitrile (also termed isocyanide) natural products exhibit potent antimicrobial activities, but little is known about their molecular targets. Here, we focus on the so far neglected group of monoisonitriles to gain further insights into their antimicrobial mode of action (MoA). Screening a focused...
ORGANISM(S): Staphylococcus aureus 
2024-06-26 | PXD050437 | Pride
Antibiotics Sar Moa studies Target identification Isocyanide Covalent inhibitors 
ProteomicsLight-activatable, 2,5-disubstituted tetrazoles for the proteome-wide profiling of aspartates and glutamates in living bacteria
Covalent inhibitors have recently seen a resurgence of interest in drug development. Nevertheless, compounds, that do not rely on an enzymatic activity, have almost exclusively been developed to target cysteines. Expanding the scope to other amino acids would be largely facilitated by the ability to...
ORGANISM(S): Escherichia coli Staphylococcus aureus 
2020-04-14 | PXD016659 | Pride
Covalent inhibitors Antibiotics Chemoproteomics Isodtb-abpp 
ProteomicsSulfamate acetamides as self-immolative electrophiles for covalent ligand- directed release chemistry
Electrophiles for covalent inhibitors that are suitable for in vivo administration are rare. While acrylamides are prevalent in FDA approved covalent drugs, chloroacetamides are considered too reactive for such purposes. We report sulfamate-based electrophiles that maintain chloroacetamide-like geom...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD038301 | Pride
Human Chemical probes Isodtb 
UnknownA Highly Reactive Cysteine-Targeted Acrylophenone Chemical Probe That Enables Peptide/Protein Bioconjugation and Chemoproteomics Analysis.
Not available
S-EPMC12728623 | biostudies-literature
ProteomicsCompetitive Profiling of Ligandable Cysteines in Staphylococcus aureus with an Organogold Compound
With the idea of exploiting metal-templated reactions to achieve selective modification of cysteines in proteins for antibacterial applications, an organometallic cyclometalated Au(III) compound was explored in a competitive chemoproteomic approach based on the isoDTB-ABPP (isotopically labelled des...
ORGANISM(S): Staphylococcus aureus 
2022-04-19 | PXD031708 | Pride
Covalent inhibitors Organogold Competitive residue-specific proteomics Antibiotics 
ProteomicsSulfamate acetamides as self-immolative electrophiles for covalent ligand- directed release chemistry - Pull Down data
Electrophiles for covalent inhibitors that are suitable for in vivo administration are rare. While acrylamides are prevalent in FDA approved covalent drugs, chloroacetamides are considered too reactive for such purposes. We report sulfamate-based electrophiles that maintain chloroacetamide-like geom...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD038375 | Pride
Human Pull down Chemical probes 
ProteomicsA tailored phosphoaspartate probe unravels CprR as a response regulator in Pseudomonas aeruginosa interkingdom signaling
Pseudomonas aeruginosa is a difficult-to-treat Gram-negative bacterial pathogen causing life-threatening infections. Adaptive resistance (AR) to cationic peptide antibiotics such as polymyxin B impairs the therapeutic success. This self-protection is mediated by two component systems (TCS) consistin...
ORGANISM(S): Pseudomonas aeruginosa PAO1 Escherichia coli Bacillus subtilis 
2021-02-16 | PXD022426 | Pride
Response regulator P. aeruginosa Adaptive resistance Antimicrobial peptide Phosphorylation Activity-based protein profiling Parallel reaction monitoring Isotopically labeled tags B. subtilis 
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