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ProteomicsIsotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome
In the presented datasets, we use newly developed isotopically labeled desthiobiotin azide (isoDTB) tags to residue-specifically quantify cysteines in a variety of bacterial strains and a human cell line with consistently high coverage. We e.g. quantify 59% of all cysteines in essential proteins in ...
ORGANISM(S): Staphylococcus aureus 
2019-12-02 | PXD015457 | Pride
Covalent inhibitors Antibiotics Protein modifications Isotopic labeling Proteomics 
ProteomicsDegrasyn exhibits antibiotic activity against multi-resistant Staphylococcus aureus by modifying several essential cysteines
In this dataset, we investigate the targets of degrasyn in the methicillin-sensitive S. aureus strain NCTC 8325. This includes data on enrichment studies and competition studies with conventional ABPP using a degrasyn-derived probe, competition studies with residue-specific proteomics using the isoD...
ORGANISM(S): Staphylococcus aureus 
2020-04-14 | PXD016413 | Pride
Chemoproteomics Antibioitcs S. aureus Isodtb-abpp 
UnknownIsotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome.
Not available
S-EPMC7027453 | biostudies-literature
ProteomicsisoDTB-ABPP Cysteine Chemoproteomic Profiling for bigelovin
isoDTB-ABPP Cysteine Chemoproteomic Profiling was used to detect the targets of bigelovin in NLRP3 inflammasome activation. LPS and ATP induced bone marrow derived macrophages were adopted for the experiment in three biological replicates. Cell was treated with DMSO and bigelovin respectively. The c...
ORGANISM(S): Mus Musculus 
2024-06-11 | PXD053000 |
ProteomicsCompetitive Profiling of Ligandable Cysteines in Staphylococcus aureus with an Organogold Compound
With the idea of exploiting metal-templated reactions to achieve selective modification of cysteines in proteins for antibacterial applications, an organometallic cyclometalated Au(III) compound was explored in a competitive chemoproteomic approach based on the isoDTB-ABPP (isotopically labelled des...
ORGANISM(S): Staphylococcus aureus 
2022-04-19 | PXD031708 | Pride
Covalent inhibitors Organogold Competitive residue-specific proteomics Antibiotics 
ProteomicsSulfamate acetamides as self-immolative electrophiles for covalent ligand- directed release chemistry
Electrophiles for covalent inhibitors that are suitable for in vivo administration are rare. While acrylamides are prevalent in FDA approved covalent drugs, chloroacetamides are considered too reactive for such purposes. We report sulfamate-based electrophiles that maintain chloroacetamide-like geom...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD038301 | Pride
Human Chemical probes Isodtb 
ProteomicsLight-activatable, 2,5-disubstituted tetrazoles for the proteome-wide profiling of aspartates and glutamates in living bacteria
Covalent inhibitors have recently seen a resurgence of interest in drug development. Nevertheless, compounds, that do not rely on an enzymatic activity, have almost exclusively been developed to target cysteines. Expanding the scope to other amino acids would be largely facilitated by the ability to...
ORGANISM(S): Escherichia coli Staphylococcus aureus 
2020-04-14 | PXD016659 | Pride
Covalent inhibitors Antibiotics Chemoproteomics Isodtb-abpp 
ProteomicsTargeted Protein Localization by Covalent 14-3-3 Recruitment
chemoproteomic profiling of covalent ligands that target 14-3-3
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-08-28 | PXD054696 | Pride
14-3-3 
ProteomicsIsocyanides inhibit bacterial pathogents by covalent targeting of essential metabolic enzymes
Isonitrile (also termed isocyanide) natural products exhibit potent antimicrobial activities, but little is known about their molecular targets. Here, we focus on the so far neglected group of monoisonitriles to gain further insights into their antimicrobial mode of action (MoA). Screening a focused...
ORGANISM(S): Staphylococcus aureus 
2024-06-26 | PXD050437 | Pride
Antibiotics Sar Moa studies Target identification Isocyanide Covalent inhibitors 
ProteomicsScreening privileged alkylguanidinium motifs under host-mimicking conditions reveals a novel antibiotic with an unconventional mode of action
Screening large molecule libraries against pathogenic bacteria is often challenged by a low hit rate due to limited uptake, underrepresentation of antibiotic structural motifs and assays which do not resemble the infection conditions. To address these limitations, we here screen a library focused on...
ORGANISM(S): Staphylococcus aureus 
2024-07-17 | PXD051986 | Pride
Antibiotics Chemical proteomics Sar Moa studies Guanidinium 
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