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ProteomicsProbing the HIV-Host Interactome in Infected Cells
Although genetically compact, HIV-1 commandeers vast arrays of cellular machinery that sustain and protect it during cycles of viral outgrowth. Transposon-mediated saturation linker scanning mutagenesis was used to isolate fully replication-competent viruses harboring a potent foreign epitope tag. U...
ORGANISM(S): Homo sapiens (Human) 
2016-07-06 | PXD003762 | Pride
Protein interactions Ap-ms Env I-dirt Vif 
ProteomicsExploring How Workflow Variations in Denaturation-Based Assays Impact Global Protein-Protein Interaction Predictions
Denaturation-based assays such as thermal proximity coaggregation (TPCA) and ion-based proteome-integrated solubility alteration (I-PISA) are powerful tools for mapping global protein-protein interaction (PPI) networks. These workflows utilize different denaturation methods to probe PPIs, however, h...
ORGANISM(S): Homo sapiens (Human) 
2026-02-02 | PXD058773 | Pride
Human Tpca I-pisa Tpp 
ProteomicsThe Protein Composition of Human Adenovirus Replication Compartments.
Human adenoviruses are double–stranded DNA viruses that replicate in the cell nucleus and induce the formation of replication compartments (RCs) that are critical in viral replication and control of virus-host interactions. RCs are specialized virus-induced subnuclear microenvironments in which vira...
ORGANISM(S): Homo sapiens (Human) 
2024-11-20 | PXD051745 | Pride
Viral replication compartment (rc) Hmgb1 Human adenovirus (hadv) Biomolecular condensates (bmcs) Histone chaperones Tandem mass tag-based quantitative mass spectrometry 
Proteomics,MultiomicsInteractome analysis of PYHIN immune regulators
The human PYHIN proteins, AIM2, IFI16, IFIX, and MNDA, are regulators of immune response, transcription, apoptosis, and cell cycle. However, their protein interactions and underlying mechanisms remain largely uncharacterized. Here, we provide the interaction network for all PYHIN proteins. By design...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-20 | MSV000079955 | MassIVE
ProteomicsA Bifunctional SPD-0090 contributes virulence of Streptococcus pneumoniae by affecting iron transport and galactose metabolism
Streptococcus pneumoniae (S.pneumoniae) is a Gram-positive bacterial pathogen that colonizes on the mucosal surfaces of the host's nasopharynx and upper respiratory tract and results in pneumonia. In order to survive and infect the host, S.pneumoniae must have obtain essential nutrients, such as tra...
ORGANISM(S): Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) 
2022-06-01 | PXD030183 | Pride
Spd-0090 Virulence Iron transport protein Galactose metabolism Streptococcus pneumoniae 
TranscriptomicsHNRPA2B1 HITS-CLIP
Expression, crosslinking and immunoprecipitation of tagged HNRPA2B1 (variant A2) to identify bound RNA sequences RNA molecules that are immunoprecipitated are sequenced followed purification under denaturing conditions
ORGANISM(S): Homo sapiens 
2012-02-28 | E-GEOD-35799 | biostudies-arrayexpress
ProteomicsAP-MS analysis of sirtuin-4 interactions in human fibroblasts
Sirtuins (SIRTs) form a critical family of nicotinamide adenine dinucleotide (NAD)-dependent enzymes that govern genome regulation, metabolism, and aging. Despite conserved deacetylase domains, SIRTs4-7 have little to no deacetylase activity, and a robust catalytic activity for mitochondrial SIRT4 h...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-04 | MSV000079557 | MassIVE
ProteomicsAP-MS analysis of human histone deacetylase interactions in CEM-T cells
Affinity purification-mass spectrometry (AP-MS) datasets of eleven human histone deacetylases (HDACs). Affinity-tagged (GFP) HDACs were expressed in a human CEM-T cell line. For label-free analysis, proteins were digested by trypsin in-solution and peptides separated across a linear 180 min gradient...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-07 | MSV000079563 | MassIVE
ProteomicsExploring How Workflow Variations in Denaturation-Based Assays Impact Global Protein-Protein Interaction Predictions (Influenza A Data)
Denaturation-based assays such as thermal proximity coaggregation (TPCA) and ion-based proteome-integrated solubility alteration (I-PISA) are powerful tools for mapping global protein-protein interaction (PPI) networks. These workflows utilize different denaturation methods to probe PPIs, however, h...
ORGANISM(S): Homo sapiens (Human) Influenza A virus 
2026-02-02 | PXD068620 | Pride
Tpca Infuneza a 
ProteomicsAP-MS analysis of sirtuin-2 interactions and whole-cell proteome analysis in human fibroblasts
Sirtuins are NAD+-dependent deacylases with diverse enzymatic activities and substrates. They were implicated in regulation of cancer progression, neurodegeneration, aging, and viral infection. Sirtuin-2 localizes to the cytoplasm and is known to deacetylate alpha-tubulin. Current knowledge on sirtu...
ORGANISM(S): Homo sapiens (Human) 
2016-08-12 | PXD004212 | Pride
Human fibroblasts Lc-ms/ms 
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