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UnknownIdentification of Broad-Spectrum MMP Inhibitors by Virtual Screening.
Not available
S-EPMC8347235 | biostudies-literature
ProteomicsExploring How Workflow Variations in Denaturation-Based Assays Impact Global Protein-Protein Interaction Predictions
Denaturation-based assays such as thermal proximity coaggregation (TPCA) and ion-based proteome-integrated solubility alteration (I-PISA) are powerful tools for mapping global protein-protein interaction (PPI) networks. These workflows utilize different denaturation methods to probe PPIs, however, h...
ORGANISM(S): Homo sapiens (Human) 
2026-02-02 | PXD058773 | Pride
Human Tpca I-pisa Tpp 
ProteomicsImproved drug-target deconvolution with PISA-DIA using an extended, overlapping temperature gradient
For target-engagement applications, a comparative analysis was performed to assess the suitability, ease of implementation and cost-effectiveness between Cellular Thermal Shift Assay (CETSA) and Proteome Integral Solubility Alteration (PISA). Human multiple myeloma cells U266B1 cell lysate was treat...
ORGANISM(S): Homo sapiens (Human) 
2024-05-20 | PXD044545 | Pride
Data independent acquisition (dia) Thermal proteome profiling Proteomics Mass spectrometry Target engagement Protein stability Proteome integral solubility alternation 
ProteomicsIon-based Proteome Integrated Solubility Alteration Assay for System-wide Profiling of Protein-Molecule Interactions - full curve data
Unbiased drug target engagement deconvolution and mechanism of action elucidation are major challenges in drug development. Modification-free target engagement methods, such as thermal proteome profiling, have gained increasing popularity over the last several years. However, these methods have limi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD033081 | Pride
Proteomics Mechanism of action Drug target deconvolution Thermal protein profiling Cancer 
ProteomicsIon-based Proteome Integrated Solubility Alteration Assay for System-wide Profiling of Protein-Molecule Interactions
Unbiased drug target engagement deconvolution and mechanism of action elucidation are major challenges in drug development. Modification-free target engagement methods, such as thermal proteome profiling, have gained increasing popularity over the last several years. However, these methods have limi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD030695 | Pride
Proteomics Mechanism of action Drug target deconvolution Thermal protein profiling Cancer 
UnknownIon-Based Proteome-Integrated Solubility Alteration Assays for Systemwide Profiling of Protein-Molecule Interactions.
Not available
S-EPMC9118197 | biostudies-literature
UnknownCo-infection with SARS-CoV-2 omicron BA.1 and BA.2 subvariants in a non-vaccinated woman.
Not available
S-EPMC9129255 | biostudies-literature
UnknownSynthesis of morphologically diverse dual thermo- and pH-responsive nano-objects <i>via</i> RAFT-mediated emulsion PISA.
Not available
S-EPMC12584748 | biostudies-literature
ProteomicsProteome Integral Solubility Alteration assay in zebrafish embryo for exploring exposure to bioactive compounds including pollutants and chemicals mixtures
The One Health initiative connects human and animal health with their shared environment. The development of novel methodology to unbiased identification of mechanisms of action of chemicals and chemicals mixtures would address one of today´s challenges of environmental and human toxicology. Zebrafi...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2022-02-28 | PXD021670 | Pride
Thermal proteome profiling Bioactive compound Pollutants Drug target Zebrafish embryo Biodiscovery Novel drug Proteome integral solubility alteration assay Chemical mixtures 
UnknownPhosphatidylinositol-3-kinase α catalytic subunit gene somatic mutations in bronchopulmonary neuroendocrine tumours.
Not available
S-EPMC3583575 | biostudies-literature
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