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ProteomicsCryo-slicing BN-MS of rat brain mitochondrial proteins
Blue-native gel electrophoresis (BN) is a powerful method for protein separation. Combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) it enables large-scale identification of protein complexes and their subunits. Current BN-MS approaches, however, are limited in size resolution, ...
ORGANISM(S): Rattus norvegicus (Rat) 
2015-12-01 | PXD002681 | Pride
Cryo-slicing bn-ms lc-ms/ms complexome proteine profiles 
UnknownCryo-slicing Blue Native-Mass Spectrometry (csBN-MS), a Novel Technology for High Resolution Complexome Profiling.
Not available
S-EPMC4739680 | biostudies-literature
ProteomicsHigh-Resolution Complexome-Profiling of Mitochondria Reveals Quality Control Pathways of Protein Import
Mitochondria contain ~1,000 different proteins that may assemble into larger entities such as respiratory (super)complexes and preprotein translocases. The molecular/structural organization of major parts of the mitochondrial proteome, however, has not yet been resolved. We report a quantitative map...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-02-03 | PXD029548 | Pride
Complexome Lc-ms Bn-ms Cryo-slicing Mitochondria Bn-page Yeast 
ProteomicsC-terminal-dependent control of EAAT2 signaling, corticostriatal synaptic glutamate clearance and spontaneous motor activity in mice with hypokinesia
Deficiency of the astrocytic excitatory amino acid transporter type 2 (EAAT2) and, consequently, inefficient glutamate clearance at corticostriatal synapses may contribute to the depression of self-initiated movements in HuntingtonÕs disease (HD). Here we report that the removal of the last 68 amino...
ORGANISM(S): Mus musculus (Mouse) 
2022-01-26 | PXD029194 | Pride
Huntington's disease  eaat2 interaction proteomics  hypokinesia  corticostriatal synaptic transmission  glutamate clearance  synaptic targeting of eaat2 
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