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TranscriptomicsSingle intestinal stem cells build crypt-villus structures in vitro without a cellular niche
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of approximately six cycling Lgr5+ stem cells at the bottoms of small intestinal crypts1. We have now established long-term culture conditions under which single crypts und...
ORGANISM(S): Mus musculus 
2009-04-03 | E-GEOD-14594 | biostudies-arrayexpress
TranscriptomicsExpression data from YAP-S127A Villin-rtTA crypts
We overexpressed YAP in the intestinal epithelium by adding doxycycline to the drinking water of experimental mice. Mice were given dox for 2 days, and referred to as Tg (transgenic). Mice were given dox for 2 days, sacrificed, and crypts were isolated for RNA extraction and analyzed by Affymetrix m...
ORGANISM(S): Mus musculus 
2012-11-29 | E-GEOD-41506 | biostudies-arrayexpress
TranscriptomicsNormal colon top vs colon crypts
The study is to define the genetic programs of human colonic cell differentiation, and to identify candidate markers for colonic stem cells and stem cell niche The study assayed the expression of 9 paired normal colon top versus crypts. Set of arrays organized by shared biological context, such as o...
ORGANISM(S): Homo sapiens 
2007-08-28 | E-GEOD-6894 | biostudies-arrayexpress
TranscriptomicsRNA-seq of crypt cells from mouse small intestinal epithelium with an intestinal-epithelial specific deletion of LSD1 compared to wild type crypts
To assess the role of LSD1 in mouse small intestinal epithelium, we isolated small intestinal crypts from wild type (WT) (Villin-Cre -; Lsd1f/f) and intestinal-epithelial-specific knock-out (KO) (Villin-Cre+; Lsd1f/f) mice. We dissociated crypts into single cells, and FACS sorted Epcam+ cells, to av...
ORGANISM(S): Mus musculus 
2020-11-16 | E-MTAB-7862 | biostudies-arrayexpress
TranscriptomicsPaneth cells constitute the niche for Lgr5 stem cells in intestinal crypts
Homeostasis of self-renewing small intestinal crypts results from neutral competition between Lgr5 stem cells, small cycling cells located at crypt bottoms1, 2. Lgr5 stem cells are interspersed between terminally differentiated Paneth cells, that are known to produce bactericidal products such as ly...
ORGANISM(S): Mus musculus 
2010-11-29 | E-GEOD-25109 | biostudies-arrayexpress
ProteomicsSpatial proteomics mouse intestine
Alcohol induced damages to teh crypts and villi of mouse small intestine revealed by laser capture microdissection (LCM)-based sample collection, DIA-based untargeted proteomics
ORGANISM(S): Mus musculus (Mouse) 
2024-05-21 | PXD048687 | Pride
Alcohol Crypts Villi Dia proteomics 
TranscriptomicsExpression data of small intestine crypts and villi from mice with nutritional and genetic risk factors for intestinal tumors
Nutritional and genetic risk factors for intestinal tumors are additive on mouse tumor phenotypes, demonstrating that diet and genetic factors impact risk by distinct combinatorial mechanisms. We analyzed expression profiles of small intestine crypts and villi from mice with nutritional and genetic ...
ORGANISM(S): Mus musculus 
2011-05-31 | E-GEOD-29538 | biostudies-arrayexpress
TranscriptomicsMicroRNA miR-16 is anti-proliferative in enterocytes and exhibits diurnal rhythmicity in intestinal crypts
This is the first report to document circadian rhythmicity of specific microRNAs in rat jejunum. Our data provide a link between the anti-proliferative microRNA miR-16 and the intestinal proliferation rhythm and point to miR-16 as an important regulator of proliferation in jejunal crypts. This funct...
ORGANISM(S): Rattus norvegicus 
2010-08-13 | E-GEOD-20603 | biostudies-arrayexpress
TranscriptomicsGene expression data from DSS-treated crypts, normal colon crypts and tumor crypts
Comparative gene expression profiling between DSS-treated crypts and normal colon crypts Comparative gene expression profiling between normal colon crypts and tumor crypts
ORGANISM(S): Mus musculus 
2017-01-01 | GSE56050 | GEO
TranscriptomicsRNA-seq of intestinal crypts vs intestinal smooth muscle with a specific MMP17 deletion compared to wild-type
We wanted to assess the role of a specific smooth muscle protein (MMP17) in two different intestinal compartments, the epithelium (crypts) and the smooth muscle. To do that we isolate intestinal crypts from wild-type (WT) and knockout (KO, Mmp17-/-) mice, and obtained clean strips of smooth muscle. ...
ORGANISM(S): Mus musculus 
2021-09-27 | E-MTAB-9180 | biostudies-arrayexpress
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