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Proteomicsα-arrestin-dependent endocytosis after cycloheximide treatment
To identify new plasma membrane (PM) proteins that require α-arrestins for endocytosis, we performed an iTRAQ-based quantitative proteomic screening. We analysed and compared the PM proteome for each arrestin knockout (art∆) strain to a WT strain after a 90 min cycloheximide (CHX) treatment. CHX is ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-09-25 | PXD014695 | Pride
Endocytosis Arrestin Plasma membrane Cycloheximide 
TranscriptomicsTranscription profiling of parthenogenetic mouse embryos treated with cycloheximide or strontium chloride
gene expression pattern of parthenogenetic mouse embryos by cycloheximide or Sr2+.
ORGANISM(S): Mus musculus 
2006-06-05 | E-MNIA-85 | biostudies-arrayexpress
TranscriptomicsCHX Treatment
Cycloheximide treatment of cotton ovules Keywords: Antibiotic Treatment Single timepoint, 6 slides, 3 Biological replicates, each biological replicate having 2 technical replicates. Dyes were swapped between technical replicates.
ORGANISM(S): Gossypium hirsutum 
2005-11-03 | E-GEOD-3543 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human hormone treated MCF7 breast cancer cells in the presence or absence of cycloheximide
Estrogen receptors (ERs), which mediate the proliferative action of estrogens in breast cancer cells, are ligand-dependent transcription factors that regulate expression of their primary target genes through several mechanisms. In addition to direct binding to cognate DNA sequences, ERs can be recru...
ORGANISM(S): Homo sapiens 
2008-06-16 | E-GEOD-8597 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse T cell in response to cycloheximide treatment
Cycloheximide has been used for inhibiting protein synthesis in order to identify IL-2 direct targeted genes in T cells, but the effects of cycloheximide itself on genomewide genes are not well defined; We conducted a global analysis of cycloheximide regulated genes in mouse T cell line CTLL-2 using...
ORGANISM(S): Mus musculus 
2009-01-17 | E-GEOD-6084 | biostudies-arrayexpress
GenomicsRibosome profiling data of HEK293T cells treated with C13-benzamidyl cycloheximide
Ribosome profiling data of HEK293T cells treated with C13-benzamidyl cycloheximide
PRJNA764910 | ENA
TranscriptomicsDissecting primary (translation independent) from secondary (translation dependent) IFN-mediated differential gene expression
NIH-3T3 cells were pretreated for 15 min with either DMSO (mock) or cycloheximide followed by addition of either mock, 100 U/ml IFNalpha or 100 U/ml IFNgamma for 1h. During the last 30 min, 500 µM 4-thiouridine was added to cell culture medium. Total cellular RNA was isolated using Trizol reagent an...
ORGANISM(S): Mus musculus 
2013-07-04 | E-GEOD-30457 | biostudies-arrayexpress
TranscriptomicsThe absence of a Ca(2+) signal during mouse egg activation can affect development and gene expression patterns
The absence of a Ca(2+) signal during mouse egg activation can affect parthenogenetic preimplantation development, gene expression patterns, and blastocyst quality. Gene expression pattern of parthenogenetic mouse embryos by cycloheximide or Sr2+. Keywords: development or differentiation design Gene...
ORGANISM(S): Mus musculus 
2010-06-10 | E-GEOD-5077 | biostudies-arrayexpress
GenomicsCycloheximide can distort measurements of mRNA levels and translation efficiency
Cycloheximide can distort measurements of mRNA levels and translation efficiency
PRJNA515049 | ENA
TranscriptomicsTranscription profiling of Drosophila melanogaster third instar larvae organ cultures treated with 20-hydroxyecdysone, cycloheximide vs controlds to identify 20-hydroxyecdysone regulated genes at the onset of metamorphosis
To identify 20E-regulated genes, wandering third instar larvae were dissected and their organs were cultured in the presence of either no hormone, 20E alone, cycloheximide alone, or 20E plus cycloheximide for six hours. Experiment Overall Design: Eight partial blue gut third instar larvae were disse...
ORGANISM(S): Drosophila melanogaster 
2007-10-31 | E-GEOD-3060 | biostudies-arrayexpress
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