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ProteomicsIsoTOP-ABPP in Jurkat cells for cysteine reactivity profiling
Cysteine reactivity in Jurkat cell lines were assessed using IsoTOP-ABPP
ORGANISM(S): Homo sapiens (Human) 
2021-02-04 | PXD022151 | Pride
Chemoproteomics Reactivity Cyteines 
ProteomicsGlycopeptides from recombinant EBV gH, gL and gp42 with EThcD
Soluble ectodomains of Epstein Barr Virus glycoproteins gH/gL and gp42 were expressed in HEK293F cells for structural characterization by cryo electron microscopy. The purified material was additionally analyzed by LC-MS/MS after digestion with trypsin or chymotrypsin to identify N-linked glycosylat...
ORGANISM(S): Human herpesvirus 4 
2018-04-10 | PXD006403 | Pride
Glycopeptide  epstein barr virus 
ProteomicsMitoproteomics of neuronal mitochondria of EAE mice
Inflammation in the central nervous system (CNS) can impair the function of neuronal mitochondria and contributes to axon degeneration in the common neuroinflammatory disease multiple sclerosis (MS). Here we combine cell type-specific mitochondrial proteomics with in vivo biosensor imaging to dissec...
ORGANISM(S): Mus musculus (Mouse) 
2023-10-18 | PXD032363 | Pride
Mitochondria Idh3 Neurons 
ProteomicsQuantitative proteomics of CSF from the Alzheimer's disease mouse model 5xFAD
The 5xFAD mouse model was used to study the influence of amyloid pathology on the cerebrospinal fluid (CSF) proteome. Therefore, CSF of 7-months old 5xFAD and WT control mice was sampled with an improved collection protocol. Using a minute amount of 5 µL CSF, we applied data dependent and data indep...
ORGANISM(S): Mus musculus (Mouse) 
2025-04-15 | PXD053568 | Pride
Cerebrospinal fluid Dia 5xfad Alzheimer's disease Csf Dda 
ProteomicsN-linked glycosylation of recombinant Porcine Delta Coronavirus spike protein ectodomain from drosophila S2 cells
Recombinant PDCoV spike glycoprotein was produced from Drosophile S2 cells for structural characterization by cryo electron microscopy and mass spectrometry. MS was used to assess the N-linked glycosylation profile of the purified material. We used a multiple protease digestion in combination with R...
ORGANISM(S): Deltacoronavirus PDCoV/USA/Illinois121/2014 
2017-10-24 | PXD007107 | Pride
Virus  n-linked glycosylation 
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