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Proteomicsi.e., Human HEK-293F cell line AP-MS/MS analysis of oxidative modifications in SPATA5.
The objective of this project was to identify oxidative modifications of the SPATA5 protein in C672 cells through proteomic analysis. This involved the affinity purification of SPATA5 under overexpression conditions, followed by detailed proteomic analysis to characterize the oxidative modifications...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD054917 | Pride
Hek-293f Ap-msms I. e. human 
ProteomicsTunable activated esters for lysine labeling in proteome
The development of various heteroaromatic activated esters designed for selective lysine labeling within the proteome for profiling purposes. Through systematic optimization of the reactivity and selectivity of these esters, we identified a series of effective probe molecules suitable for both in vi...
ORGANISM(S): Homo Sapiens 
2024-04-09 | PXD051323 |
ProteomicsPTMC_The proteomic profiles of pyridinium-based strategy for lysine-selective protein modification in live cells
A new bioorthogonal lysine-labeling strategy based on cationic-pyridinium activated ester, which renders the modification of peptides and proteins’ lysine residues with ultrafast kinetics and excellent specificity under aqueous solution, and profiled reactive-lysine proteome in vitro and in live cel...
ORGANISM(S): Homo Sapiens 
2023-10-24 | PXD036388 |
ProteomicsPTMC_A pyridinium-based strategy for bioorthogonal conjugation-assisted purification method for profiling cell surface proteome
Cell surface proteins (CSPs) are valuable targets for therapeutic agents, but achieving highly selective CSP enrichment in cellular physiology remains a technical challenge. To address this challenge, we propose a newly developed sulfo-pyridinium-ester (SPE) cross-linking probe, followed by two-step...
ORGANISM(S): Homo Sapiens 
2023-08-11 | PXD044541 |
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