Sort   by:  
 Page size 
ProteomicsThe ULK1 effector BAG2 regulates autophagy initiation by modulating AMBRA1 localization
In this projectby two sets of interaction-proteomic experiments were performed: (1) miniTURBO proximity labeling (PL) of ULK1 complex members ULK1, ATG13, ATG101 and RB1CC1/FIP200; and (2) anti-HA affinity purification (AP) of ULK1 complex members ULK1, ATG13, ATG101 and RB1CC1/FIP200
ORGANISM(S): Homo sapiens (Human) 
2024-08-23 | PXD044974 | Pride
Lc-msms Autophagy Kinase Human Interactome 
ProteomicsIn-depth data independent acquisition-mass spectrometry proteomic analysis of juvenile dermatomyositis serum samples reveals protein expression changes associated with muscle-specific autoantibodies
We analyzed sera from five JDM patients, each with three MSAs (anti-melanoma differentiation-associated protein 5 antibodies; anti-MDA5, anti-nuclear matrix protein 2 antibodies; anti-NXP2, or anti-transcriptional intermediary factor 1 alpha and/or gamma subunits antibodies; anti-TIF-1γ) and five he...
ORGANISM(S): Homo Sapiens (human) 
2023-04-23 | PXD036960 | JPOST Repository
ProteomicsThe role of the AP-1 adaptor complex in outgoing and incoming membrane trafficThe role of the AP-1 adaptor complex in outgoing and incoming membrane traffic
The AP-1 adaptor complex is found in all eukaryotes, but it has been implicated in different pathways in different organisms. To look directly at AP-1 function, we generated stably transduced HeLa cells co-expressing tagged AP-1 and various tagged membrane proteins. Live cell imaging showed that AP-...
ORGANISM(S): Homo sapiens (Human) 
2024-04-03 | PXD050449 | Pride
Ap-1 Silac Ip-ms Trafficking 
ProteomicsA Spatial Interactome Reveals the Protein Organization of the Algal CO2 Concentrating Mechanism
One-third of global CO2 fixation is mediated by eukaryotic algae. Nearly all algae enhance their carbon assimilation by operating a CO2 concentrating mechanism (CCM), built around a mysterious organelle called the pyrenoid. Here, we leveraged new tools to determine the localizations of 146 candidate...
ORGANISM(S): Chlamydomonas reinhardtii 
2018-01-24 | PXD007664 | Pride
Co2-concentrating mechanism Ccm Carbon-fixation Photosynthesis Pyrenoid 15n isotopic labelling Rubisco High-throughput fluorescence protein tagging Orbitrap Affinity purification mass spectrometry Chlamydomonas reinhardtii Interactome 
ProteomicsInteractome of Nematostella GW182 in HEK293 cells
Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translat...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD004668 | Pride
Ap-ms Lc-msms Gw182 Hek293 
ProteomicsRegulation of FNIP1 phosphorylation
In this project TBK1 and/or ULK1 phosphosites on FNIP1 were analyzed.
ORGANISM(S): Homo sapiens (Human) 
2026-09-25 | PXD063015 | Pride
Human Ap-ms Lc-msms Phosphorylation 
ProteomicsStructural insightsStructural insights into regulation of the PEAK3 pseudokinase scaffold by 14-3-3 into regulation of the PEAK3 pseudokinase scaffold by 14-3-3
The three members of the PEAK family of pseudokinases (PEAK1, PEAK2, and PEAK3) are molecular scaffolds that have recently emerged as important nodes in signaling pathways that control cell migration, morphology, and proliferation, and are increasingly found mis-regulated in human cancers. While no...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD035574 | Pride
Human Ap-ms Lc-msms Peak3 
ProteomicsMorphological and genetic screens reveal mechanisms of BiDAC-induced plasma membrane protein degradation
The discovery of bifunctional degradation activating compounds (BiDACs) has led to the development of a new class of drugs that promote the clearance of their protein targets. BiDAC-induced ubiquitination is generally believed to direct cytosolic and nuclear proteins to proteolytic destruction by pr...
ORGANISM(S): Homo sapiens (Human) 
2025-05-11 | PXD057365 | Pride
Human Lc-msms Ap-ms Q exactive 
Proteomicsi.e., Human HEK-293F cell line AP-MS/MS analysis of oxidative modifications in SPATA5.
The objective of this project was to identify oxidative modifications of the SPATA5 protein in C672 cells through proteomic analysis. This involved the affinity purification of SPATA5 under overexpression conditions, followed by detailed proteomic analysis to characterize the oxidative modifications...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD054917 | Pride
Hek-293f Ap-msms I. e. human 
ProteomicsNuclear versus cytoplasmic VHL interactomes
Protein misfolding is linked to a wide variety of human disease. Protective cellular protein quality control (PQC) mechanisms evolved to selectively recognize misfolded proteins and limit their toxic effects. Using yeast as a model system, we previously discovered that the misfolded protein VHL inte...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-11-04 | PXD010660 | Pride
Lc-msms Ap-ms Yeast Silac Orbitrap 
Sort   by:  
 Page size