Sort   by:  
 Page size 
no omics definedRNAi knock down of GW182 in Drososphila cells compared to cells with knock down of AGO1, a key effector of miRNA-mediated gene silencing
RNA silencing pathways are conserved gene regulation mechanisms that lead to degradation, translational repression or transcriptional silencing of target-transcripts selected based on complementarity with small RNA molecules. Using firefly luciferase reporters, we previously were able show that the ...
ORGANISM(S): Drosophila melanogaster 
2006-10-31 | E-MEXP-487 | biostudies-arrayexpress
ProteomicsInteractome of Nematostella GW182 in HEK293 cells
Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translat...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080764 | MassIVE
ProteomicsInteractome of Nematostella GW182 in HEK293 cells
Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translat...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD004668 | Pride
Ap-ms Lc-msms Gw182 Hek293 
TranscriptomicsExpression data from Tnrc6a (GW182) mutant yolk sac
GW182 (Tnrc6a) is a key component of RISC (miRNA-Induced Silencing Complex) that plays a critical role in miRNA-mediated gene silencing. Here, we show that GW182 is expressed in the yolk sac endoderm, and that gene-trap disruption of GW182 leads to growth arrest of yolk sac endoderm, impaired hemato...
ORGANISM(S): Mus musculus 
2011-08-31 | E-GEOD-30244 | biostudies-arrayexpress
GenomicsDefining an evolutionarily conserved role of GW182 in circular RNA degradation.
Defining an evolutionarily conserved role of GW182 in circular RNA degradation.
PRJNA545946 | ENA
TranscriptomicsExpression data from Tnrc6a (GW182) mutant E18.5 lungs
GW182 (Tnrc6a) is a key component of RISC (miRNA-Induced Silencing Complex) that plays a critical role in miRNA-mediated gene silencing. Here, we show that GW182 is expressed in airway multiciliated cells (MCCs), and that gene-trap disruption of GW182 leads to defective multicilia formation and down...
ORGANISM(S): Mus musculus 
2017-05-01 | GSE89327 | GEO
TranscriptomicsExpression data from Tnrc6a (GW182) mutant yolk sac
GW182 (Tnrc6a) is a key component of RISC (miRNA-Induced Silencing Complex) that plays a critical role in miRNA-mediated gene silencing. Here, we show that GW182 is expressed in the yolk sac endoderm, and that gene-trap disruption of GW182 leads to growth arrest of yolk sac endoderm, impaired hemato...
ORGANISM(S): Mus musculus 
2011-09-01 | GSE30244 | GEO
ProteomicsMapping the GW182 CIM1 binding site on CNOT1(800-999) by HDX-MS
HDX-MS experiments were performed as described in detail in Białobrzewski M.K., Cieplak-Rotowska M.K., et al (https://www.biorxiv.org/content/10.64898/2026.07.16.738963v1) on human CNOT1 (residues 800-999) in the presence of peptides corresponding to GW182 CIM1 (QSRLPQWTHP), N-CIM1 (DPSQSQSRLPQWTHPN...
ORGANISM(S): Homo sapiens (Human) 
2026-08-20 | PXD082855 | Pride
Cnot1 Hdx-ms 
TranscriptomicsDefining an evolutionarily conserved role of GW182 in circular RNA degradation.
By using dsRNA screening, we found that GW182 is critical for the degradation of circRNA in the Drosophila cells. To further confirm our finding genome widely, S2 cells were treated with either β-gal dsRNA (as a control) or GW182 dsRNA for 3 days and total RNA was extracted and sequenced after ribos...
ORGANISM(S): Drosophila melanogaster 
2019-07-16 | GSE132100 | GEO
UnknownThe C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing.
Not available
S-EPMC2673069 | biostudies-literature
Sort   by:  
 Page size