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ProteomicsAPEX proximity labeling of murine NDUFS4 interacting proteins in podocytes
In this project we used an unbiased approach to identify the proteins that interact with NDUFS4 in the mouse podocytes using the APEX2 proximity labeling system. An immortalized murine podocyte was permanently transduced with a doxycycline (DOX) inducible NDUFS4-APEX2 chimeric construct. The cells w...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-08 | PXD041378 | Pride
Apex proximity labeling of murine ndufs4 interacting proteins in podocytes 
ProteomicsMurine podocyte mitochondrial complexsome analysis
Proximity labeling discovered a few mitochondrial cristae organizing proteins as NDUFS4 interacting protein. However proximity labeling is unable to differentiate between a direct interaction or a mere close association between NDUFS4 and cristae forming proteins. Additionally, it does not specify i...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-08 | PXD041203 | Pride
Complexsome Podocyte Mouse Respiratory supercomplex 
ProteomicsMitochondria proteomics of murine kidney podocytes
To understand how diabetes alters the protein subunits of mitochondrial Electron Transfer Chain (ETC) in the podocytes, we performed a proteomic analysis of mitochondrial proteins isolated from primary kidney podocytes of WT (C57BL/6J background) and Ins2Akita/+ diabetic (C57BL/6J background) mice.
ORGANISM(S): Mus musculus (Mouse) 
2024-02-08 | PXD041202 | Pride
Primary podocyte Mitochondria Mouse Proteome 
ProteomicsMitochondria proteomics of murine kidney podocytes
To understand how diabetes alters the protein subunits of mitochondrial Electron Transfer Chain (ETC) in the podocytes, we performed a proteomic analysis of mitochondrial proteins isolated from primary kidney podocytes of WT (C57BL/6J background) and Ins2Akita/+ diabetic (C57BL/6J background) mice.
ORGANISM(S): Mus musculus (Mouse) 
2024-02-08 | PXD045828 | Pride
Primary podocyte Mitochondria Mouse Proteome 
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