Sort   by:  
 Page size 
ProteomicsUnravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine
Mass spectrometry-based proteomic analysis of urinary EV (uEV) in men with benign and malignant prostate disease, profiling the proteome of EV separated from prostate tumor interstitial fluid and matched uEV, and a comparative proteomic analysis with uEV from patients with bladder and renal cancer.
ORGANISM(S): Homo sapiens (Human) 
2020-04-09 | PXD015289 | Pride
Extracellular vesicles  exosomes  separation  isolation  urine  cancer  biomarkers 
ProteomicsProduction and application of recombinant extracellular vesicles for method development, data normalization and assessment of pre-analytical variables
The diagnostic and therapeutic use of extracellular vesicles (EV) is under intense investigation and may lead to societal benefits. Reference materials are an invaluable resource for developing, improving and assessing the performance of regulated EV applications and for quantitative and objective d...
ORGANISM(S): Homo sapiens (Human) 
2023-03-13 | PXD017542 | Pride
Recombinant Urine Extracellular vesicles Human Plasma 
TranscriptomicsHuman MCF-7 breast cancer cells: control vs. treated with conditioned medium of cancer associated adipose tissue
Transcriptional profiling of human MCF-7 breast cancer cells comparing MCF-7 cells treated with control medium (DMEM/F12 + 0,5% BSA) with MCF-7 cells treated with conditioned medium of cancer-associated adipose tissue (CMCAAT) obtained from 2 breast cancer patients. Goal was to determine the effects...
ORGANISM(S): Homo sapiens 
2015-01-07 | E-GEOD-58574 | biostudies-arrayexpress
ProteomicsComparison of performances of automatic versus manual liquid handling in density-based separation of extracellular vesicles from plasma and urine
Extracellular vesicles (EV) in body fluids are extensively studied as potential biomarkers for numerous diseases. Major impediments of EV-based biomarker discovery include the manual labor, and the specificity and reproducibility of EV sample preparation. To tackle this, we present an automated liqu...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD032394 | Pride
Plasma Urine Extracellular vesicle Automatic liquid handling 
ProteomicsA preclinical platform for assessing long-term drug efficacy exploiting mechanically tunable scaffolds colonized by a three-dimensional tumor microenvironment
Background: Long-term drug evaluation heavily relies upon rodent models. Drug discovery methods to reduce animal models in oncology may include three-dimensional (3D) cellular systems that take into account tumor microenvironment (TME) cell types and biomechanical properties. Methods: In this study...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD039590 | Pride
3d cancer model  long-term  drug evaluation  pre-clinical  micro-environment  stiffness 
ProteomicsBenchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics
Extracellular vesicle (EV) analysis from blood samples is under intense investigation and holds the potential to deliver clinically meaningful biomarkers for health and disease. Technical variation must be minimized to confidently assess EV-associated biomarkers but the impact of pre-analytics on EV...
ORGANISM(S): Homo sapiens (Human) 
2023-03-13 | PXD035199 | Pride
Biomarkers  proteomics  extracellular vesicles  exosomes  serum  plasma  anticoagulants  preservatives  cancer 
TranscriptomicsHypoxia-driven alterations in the B16 melanoma cell transcriptome account for a higher metastatic potential
Hypoxia-driven alterations in the B16 melanoma cell transcriptome account for a higher metastatic potential: evidence for a role of Ero1L We analyzed transcriptomic adaptations to hypoxia/reoxygenation in B16 melanoma cells. By Ero1L over- and down-expression in vivo, we identified this ER oxidase ...
ORGANISM(S): Mus musculus 
2013-08-01 | E-GEOD-14727 | biostudies-arrayexpress
ProteomicsTargeted analysis of PSMA in prostate cancer patient urinary extracellular vesicles labeled for PSMA and separated with asymmetrical flow field-flow fractionation coupled to a fluorescent light detector
Urine of 5 prostate cancer patients was collected, and labeled for PSMA. Targeted proteomic analysis was performed on the asymmetrical flow field-flow fractionated samples.
ORGANISM(S): Homo Sapiens 
2026-03-10 | PXD031292 | panorama
TranscriptomicsExpression profiling of migrated and invaded breast cancer cells predicts early metastatic relapse and reveals KrM-CM-
Cell motility and invasion initiate metastasis. However, only a subpopulation of cancer cells within a tumor will ultimately become invasive. Due to this stochastic and transient nature, in an experimental setting, migrating and invading cells need to be isolated from the general population in order...
ORGANISM(S): Homo sapiens 
2014-01-29 | E-GEOD-54465 | biostudies-arrayexpress
TranscriptomicsCCN5, a novel transcriptional repressor
CCN5 is a member of the CCN (Connective Tissue Growth Factor/Cysteine-rich 61/Nephroblastoma overexpressed) family and was identified as an estrogen-inducible gene in estrogen receptor-positive cell lines. However, the role of CCN5 in breast carcinogenesis remains unclear. We report here that CCN5 p...
ORGANISM(S): Homo sapiens 
2011-11-30 | E-GEOD-25431 | biostudies-arrayexpress
Sort   by:  
 Page size