Sort   by:  
 Page size 
MetabolomicsMetabolomic analysis of riboswitch containing E. coli recombinant expression system
In this study we have employed metabolomics approaches to understand the metabolic effects of producing enhanced green fluorescent protein (eGFP) as a recombinant protein in Escherichia coli cells. This metabolic burden analysis was performed against a number of recombinant expression systems and co...
2017-11-16 | MTBLS393 | MetaboLights
MetabolomicsMonitoring recombinant protein expression in bacteria by rapid evaporative ionisation mass spectrometry

RATIONALE: There is increasing interest in methods of direct analysis mass spectrometry that bypass complex sample preparation steps.

METHODS: One of the most interesting new ionisation methods is rapid evaporative ionisation mass spectrometry (REIMS) in wh...

2022-01-28 | MTBLS957 | MetaboLights
MetabolomicsRecombinant Production of Biliverdin IXβ and δ Isomers in the T7 Promoter Compatible Escherichia coli Nissle
The ability to obtain purified biliverdin IX (BVIX) isomers other than the commercially available BVIXα is limited due to the low yields obtained by the chemical coupled oxidation of heme. Chemical oxidation requires toxic chemicals, has very poor BVIX yields (<0.05%), and is not conducive to scalab...
2021-12-16 | MTBLS3555 | MetaboLights
TranscriptomicsTranscription profiling of recombinant E. coli to heat-shock and dual heat-shock recombinant protein induction
Recombinant Escherichia coli cultures are used to manufacture numerous therapeutic proteins and industrial enzymes, where many of these processes use elevated temperatures to induce recombinant protein production. The heat-shock response in wild-type E. coli has been well studied. In this study, t...
ORGANISM(S): Escherichia coli 
2009-08-25 | E-GEOD-17507 | biostudies-arrayexpress
TranscriptomicsGene expression analysis of chickens (Gallus gallus) infected with recombinant influenza viruses
H5N1 subtype highly pathogenic avian influenza virus has been spreading to Asia, Eurasia and African coutries. An original or six of recombinant H5N1 subtype influenza viruses with varying survivability were infected to chickens for elucidating genes correlated with pathogenicity. Two chickens were ...
ORGANISM(S): Gallus gallus 
2012-03-30 | E-GEOD-32378 | biostudies-arrayexpress
Genomicsrecombinant coronavirus
Recombinant OC43 SARS-CoV-2 Spike Replacement Virus: An Improved BSL-2 Proxy Virus for SARS-CoV-2 Neutralization Assays
PRJNA1064152 | ENA
TranscriptomicsGeneration of Induced Pluripotent Stem Cells using Recombinant Proteins
Groundbreaking work demonstrated that ectopic expression of four transcription factors, Oct4, Klf4, Sox2, and c-Myc, could reprogram murine somatic cells to induced pluripotent stem cells (iPSCs) (Takahashi and Yamanaka, 2006), and human iPSCs were subsequently generated using similar genetic manipu...
ORGANISM(S): Mus musculus 
2010-04-09 | E-GEOD-16062 | biostudies-arrayexpress
ProteomicsRecombinant Tau and EFhd2 from Bacteria
E. coli is a commonly used host for generating large quantities of human recombinant proteins. These proteins are often considered free of post-translational modifications (PTMs); however, a subset of the produced proteins can incorporate modifications derived from the host bacterial enzymes. Here, ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-07-08 | PXD020022 | Pride
E. coli Tau Ptm Recombinant Efhd2 Lc-ms/ms 
TranscriptomicsTranscription profiling of E. coli high-cell-density recombinant and wild-type
The transcriptome profiles for wild-type (plasmid-free) and recombinant (plasmid-bearing) Escherichia coli during well-controlled synchronized high-cell-density fed-batch cultures were analyzed by DNA microarrays. It was observed that the growth phase significantly affected the transcriptome profile...
ORGANISM(S): Escherichia coli 
2009-08-25 | E-GEOD-17505 | biostudies-arrayexpress
ProteomicsRecombinant SWATH library for identification of low abundant human plasma proteins
Recombinant SWATH library for identification of low abundant human plasma proteins
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-04 | MSV000086411 | MassIVE
Sort   by:  
 Page size