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High-resolution MS/MS spectra of peptides can be deisotoped to identify monoisotopic masses of peptide fragments. The use of such masses should improve protein identification rates. However, deisotoping is not universally used and its benefits have not been fully explored. Here, we developed MS2-Dei...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-08-05 | PXD010587 | Pride
Isobaric labelling technique coupled with high resolution mass spectrometry has been widely employed in the proteomics workflows requiring relative quantification. For each high resolution tandem mass spectrum (MS/MS), it can be used not only to quantify the peptide from different samples by reporte...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2015-01-30 | PXD000994 | Pride
Previously we found that human pluripotent stem cells (hPSCs) utilize glucose differently depending on the presence of the feeder cells, which are mouse embryonic fibroblasts, or MEFs. More specifically, feeder-free cultured hPSCs are more reliant on glycolysis for proliferation. Therefore, we hypo...
ORGANISM(S): Mus musculus (Mouse) 
2022-04-01 | PXD013662 | Pride
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