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Next-generation sequencing has been widely used for the genome-wide profiling of histone modifications, transcription factor binding and gene expression through chromatin immunoprecipitated DNA sequencing (ChIP-seq) and cDNA sequencing (RNA-seq). Here, we describe a versatile library construction me...
ORGANISM(S): Mus musculus 
Methanothermobacter thermautotrophicus is a model thermophilic hydrogenotrophic methanogen. The life and survival of M. thermautotrophicus is highly influenced by the availability of nutrients and temperature. To learn about adaptation mechanisms evolved by the archaea to cope with drastic temperatu...
ORGANISM(S): Methanobacterium 
2018-10-02 | PXD006685 | Pride
In order to clarify the molecular mechanisms of drought tolerance between different maize (Zea mays L.) varieties at the protein level, iTRAQ (the isobaric tags for relative and absolute quantitation) quantitative proteomics were used for the comparative analysis of protein expression in the seedlin...
ORGANISM(S): Zea mays (Maize) 
2019-11-13 | PXD012344 | Pride
Septin proteins interact intermolecularly to form hetero-oligomeric complexes that further assemble into higher-order filaments. To investigate whether the interactions among septin proteins are affected by RID, we conducted affinity purification-mass spectrometry (AP-MS) experiments. In this regard...
ORGANISM(S): Homo sapiens (Human) 
2024-06-22 | PXD046497 | Pride
In order to study the membrane protein expression level of strain M382 in the presence or absence of thiosulfate, M382 strains were grown in marine broth 2216 with or without 10 mM thiosulfate, incubated in six-well plates at 25 ℃ for 48 hours, and perform Proteome sequencing. M382 belongs to the R...
ORGANISM(S): Alisedimentitalea scapharcae 
2023-03-26 | PXD040961 | Pride
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