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TranscriptomicsThe effect of Dis3 knockdown on miRNA expression in Drosophila wing imaginal discs.
We investigated the effect on miRNA expression in Drosophila melanogaster wing imaginal discs following the knockdown of the 3'-5' exoribonuclease Dis3.
ORGANISM(S): Drosophila melanogaster 
2015-06-01 | E-MTAB-3569 | biostudies-arrayexpress
TranscriptomicsInactivation of the exosome ribonuclease DIS3 triggers the pluripotency factor LIN28B, repressing let-7 miRNAs and unleashing MYC and RAS.
Somatic mutations affecting DIS3, the catalytic component of the RNA exosome, have been found in up to 18% of patients affected by the hematological cancer multiple myeloma. Here we show that DIS3 targets and degrades the pluripotency factor LIN28B. In cancer cells, DIS3 inactivation leads to enhanc...
ORGANISM(S): Homo sapiens 
2015-06-12 | E-GEOD-55246 | biostudies-arrayexpress
TranscriptomicsCUT&Tag for S9.6 in DIS3-depleted cells
CUT&Tag was performed to identify the effect of DIS3 depletion on DNA:RNA hybrids genome-wide. DIS3-AID HCT116 cells were treated with either Auxin (for rapid depletion of DIS3) or DMSO (control). CUT&Tag was performed targeting DNA:RNA hybrids using the CUT&Tag-IT® R-loop Assay Kit (Active Motive) ...
ORGANISM(S): Homo sapiens 
2026-08-03 | E-MTAB-17179 | biostudies-arrayexpress
TranscriptomicsCUT&Tag for RNAPII-Ser2p or RNAPII-Ser5p in DIS3-depleted cells
CUT&Tag was performed to identify the effect of DIS3 depletion on RNAPII in response to UV irradiation. DIS3-AID HCT116 cells were treated with either Auxin (for rapid depletion of DIS3) or DMSO (control) before irradiation with 8 J/m2 UVC. CUT&Tag was performed targeting RNAPII-Ser2p or RNAPII-Ser5...
ORGANISM(S): Homo sapiens 
2026-08-03 | E-MTAB-16280 | biostudies-arrayexpress
GenomicsEffect of DIS3 knockdown on circRNA expression
Effect of DIS3 knockdown on circRNA expression
PRJNA1079231 | ENA
GenomicsDepletion of DIS3 from human cell lines
Depletion of DIS3 from human cell lines
PRJNA777897 | ENA
GenomicsThe effect of DIS3 depletion on RNAPII backtracking in a human cell line
LORAX-seq (Yang et al, DOI:https://doi.org/10.1016/j.molcel.2024.01.019) was used to identify the backtracked RNA in DIS3-depleted HCT116 DIS3-AID cells. Briefly, LORAX-seq was performed according to Yang et al. (2024) with following modifications. The Pol II complexes were immunoprecipitated with α...
ORGANISM(S): Homo sapiens 
2026-08-03 | E-MTAB-16220 | biostudies-arrayexpress
ProteomicsCytoplasmic DIS3 degrades circular RNAs independently of the exosome
Circular RNAs (circRNAs) are frequently generated during splicing. Their physiological roles, however, remain largely elusive. Some specific circRNAs function as microRNA (miRNA) or RNA binding protein (RBP) sponges or encode for proteins and are actively translated. Due to the lack of accessible en...
ORGANISM(S): Homo sapiens (Human) 
2025-05-05 | PXD050442 | Pride
Exosome Circular rnas Dis3 Degradation Circrna 
GenomicsOncogenic mechanisms of DIS3 mutations in Multiple Myeloma
Oncogenic mechanisms of DIS3 mutations in Multiple Myeloma
PRJNA650522 | ENA
TranscriptomicsPronounced and Extensive Microtubule Defects in a Saccharomyces cerevisiae DIS3 Mutant
The recently proposed exozyme hypothesis posits that subunits of the RNA processing exosome assemble into structurally distinct protein complexes that function in disparate cellular compartments and RNA metabolic pathways. Here, in a genetic test of this hypothesis, we examine the role of Dis3 -- a...
ORGANISM(S): Saccharomyces cerevisiae 
2013-01-01 | E-GEOD-29018 | biostudies-arrayexpress
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