Sort   by:  
 Page size 
Escherichia coli is the most widely used bacterial model organism and is the most commonly used host for the expression of recombinant proteins. Here we describe an unexpected protein translocation phenomenon in E. coli, whereby over-expression of recombinant proteins leads to high-level lysis-indep...
ORGANISM(S): Escherichia coli 
2018-03-02 | PXD006474 | Pride
In this study we have employed metabolomics approaches to understand the metabolic effects of producing enhanced green fluorescent protein (eGFP) as a recombinant protein in Escherichia coli cells. This metabolic burden analysis was performed against a number of recombinant expression systems and co...
2017-11-16 | MTBLS393 | MetaboLights
To determine the direct promoter targets of Fnr proteins, we correlated transcriptional changes observed in single fnr1 and fnr3 mutants (E-MTAB-5741) with ChIP-Seq analysis, taking advantage of C-terminally 3xFlag fnr alleles engineered into the H. seropedicae genome. ChIP-seq data were obtained fr...
ORGANISM(S): Herbaspirillum seropedicae 
Cholesterol and phosphoinositides (PI) are two critically important lipids that are found in cellular membranes and dysregulated in many disorders. Therefore, uncovering molecular pathways connecting these essential lipids may offer new therapeutic insights. We report that loss of function of lysoso...
2021-09-15 | MTBLS2612 | MetaboLights
Sort   by:  
 Page size