Transcriptomic analysis of a dne1-1 mutant, two overexpressors of DNE1 (P35S-GFP::DNE1), an overexpressor of DNE1_D153N (35S:GFP-DNE1_D153N), and vcs-6. Biological triplicates of 14 days old seedlings were analyzed.
To gain insight into the functional role of DNE1 in cytoplasmic mRNA decay, we performed Parallel Analysis of RNA Ends (PARE) and Global Mapping of Uncapped and Cleaved Transcripts (GMUCT) analysis to identify DNE1-dependent cleavage sites based on their sensitivity to XRN4 within the Arabidopsis tr...
In this work, we used an unbiased approach coupling immunoprecipitations (IPs) and mass spectrometry to define the interactome of the decapping activator DCP1 and the decapping enzyme DCP2. In addition we unravel the interactome of the endonuclease DNE1 harboring an endoribonuclease domain of the NY...
