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TranscriptomicssiRNA profiling of human H157 non-small cell lung carcinoma cells with and without DJ-1 expression knocked-down
DJ-1 expression protects H157 cells from cell death, using siRNA oligomers we knocked down DJ-1 and evaulated gene expression using Affymetrix U133A GeneChip arrays. Experiment Overall Design: H157 cells were transfected every 24 hours with siRNA oligomers for 3 consecutive days, lysed 96 hours afte...
ORGANISM(S): Homo sapiens 
2008-06-13 | E-GEOD-5519 | biostudies-arrayexpress
TranscriptomicsRNAseq of small intestinal organoids from mice treated with the cytokines IL-13 and IL-22 for 72 hours in combination with the BMP signaling inhibitor DMH1
The intestinal epithelium is our first line of defense against infections of the gut and the plasticity in cellular differentiation of the intestinal epithelium is an important part of this response. The changes in cellular composition is driven by immune cell derived cytokines. Here we use signatur...
ORGANISM(S): Mus musculus 
2022-05-08 | E-MTAB-9185 | biostudies-arrayexpress
TranscriptomicsInhibition of endogenous MTF-1 signaling in zebrafish embryos identifies novel roles for MTF-1 in development
The MTF-1 transcription factor is considered to be a master regulator of zinc homoestasis. We previously characterized a constitutively nuclear, dominant-negative zebrafish MTF-1/eGFP fusion protein (dnMTF-1). In this study, in vitro transcribed dnMTF-1 mRNA was microinjected into zebrafish embryos ...
ORGANISM(S): Danio rerio 
2014-04-25 | E-GEOD-51298 | biostudies-arrayexpress
TranscriptomicsRNA-seq of murine small intestinal organoids treated with type I PRMT inhibitor MS023 against untreated controls
Murine small intestinal organoids were cultured in the presence or absence of 5µM inhibitor MS023 (PubChem CID 92136227), which targets type I protein arginine methyltransferases Prmt1, Prmt3, Prmt4, Prmt6, Prmt8. Organoids were grown in culture media containing EGF, Noggin and R-spondin (ENR), medi...
ORGANISM(S): Mus musculus 
2020-12-16 | E-MTAB-9291 | biostudies-arrayexpress
ProteomicsQuantitative proteomic screen identifies Annexin A2 as a host target for Salmonella pathogenicity island-2 effectors SopD2 and PipB2
In this work we use stable isotope labeling of amino acids in cell culture (SILAC) and a S. Typhimurium mutant that secretes increased amounts of effectors to identify cognate effector binding partners during infection. Using this method, we identified the host protein annexin A2 as a binding partne...
ORGANISM(S): Homo sapiens (Human) Salmonella enterica subsp. enterica serovar Typhimurium str. 5579 Salmonella enterica subsp. enterica serovar Typhimurium str. A130 
2022-02-17 | PXD025582 | Pride
Proteomics Silac Salmonella 
TranscriptomicsRNA-seq of murine small intestinal organoids treated with Ep300/Crebbp inhibitor I-CBP112 against untreated controls
Murine small intestinal organoids were cultured in the presence or absence of 3µM inhibitor I-CBP112 (PubChem CID 90488984), which targets E1A Binding Protein P300 (Ep300) and Creb-binding protein (Crebbp, Cbp). Organoids were grown in culture media containing EGF, Noggin and R-spondin (ENR), media ...
ORGANISM(S): Mus musculus 
2020-12-16 | E-MTAB-9290 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of zebrafish embryos with dpf3 knocked down for morpholino
An experiment was performed to analyze the effect of knockdown of dpf3 during zebrafish embryogenesis.Morpholino against dpf3 and control morpholino were injected into eggs and eggs were kept under standard conditions for 72 hours. Embroys were harvested, total RNA was extracted and used for microar...
ORGANISM(S): Danio rerio 
2008-08-01 | E-TABM-354 | biostudies-arrayexpress
ProteomicsReduced circulating insulin enhances insulin sensitivity in old mice and extends lifespan
The causal relationships between insulin levels, insulin resistance, and longevity are not fully elucidated. Genetic down-regulation of insulin/insulin-like growth factor 1 (Igf1) signaling components can extend invertebrate and mammalian lifespan, but insulin resistance, a natural form of decreased...
ORGANISM(S): Mus musculus (Mouse) 
2017-07-07 | PXD006613 | Pride
Insulin resistance Hyperinsulinemia Longevity Aging 
TranscriptomicsIntestinal myofibroblast vs skin fibroblast
Pericryptal myofibroblasts in the colon and rectum play an important role in regulating the normal colorectal stem cell niche and facilitating tumour progression. Myofibroblasts have previously mostly been distinguished from normal fibroblasts only by the expression of α smooth muscle actin (αSMA)...
ORGANISM(S): Homo sapiens 
2016-04-06 | E-GEOD-77474 | biostudies-arrayexpress
TranscriptomicsThe important role of GPR84 in MLL leukemogenesis
Beta-catenin signaling is required for establishment of leukemic stem cells (LSCs) in acute myeloid leukemia (AML), yet the upstream regulators that can augment this pathway are unknown. Through genome-wide gene expression analysis and functional studies, we identified an important role for GPR84 in...
ORGANISM(S): Mus musculus 
2014-10-15 | E-MTAB-1932 | biostudies-arrayexpress
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