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ProteomicsMolecular basis of F-actin regulation and sarcomere assembly via myotilin
Sarcomeres, the basic contractile units of striated muscle cells, contain arrays of thin (actin) and thick (myosin) filaments that slide past each other during contraction. The Ig-like domain containing protein myotilin provides structural integrity to Z-discs—the boundaries between adjacent sarcome...
ORGANISM(S): Homo sapiens (Human) 
2021-02-22 | PXD024301 | Pride
Sarcomeric z-disc  f-actin binding  myotilin  α-actinin-2  integrative structural model of f-actin:myotilin  competitive tropomyosin binding  z-disc-assembly regulation Cross-linking mass spectrometry Lc-ms/ms 
ProteomicsBacterial stigmasterol degradation involving radical flavin delta-24 desaturase and molybdenum-dependent C26 hydroxylase
Sterols are ubiquitous membrane constituents that persist to a large extent in the environment due to their water insolubility and chemical inertness. Recently, an oxygenase-independent sterol degradation pathway was discovered in a cholesterol-grown denitrifying bacterium Sterolibacterium (S.) deni...
ORGANISM(S): Sterolibacterium denitrificans 
2024-05-21 | PXD048763 | Pride
Anaerobic metabolism Molybdenum enzyme Steroid degradation Flavin radical enzyme Stigmasterol Desaturasem 
UnknownNuclear rupture in confined cell migration triggers nuclear actin assembly to limit chromatin leakage
Not available
S-SCDT-10_1038-S44318-025-00566-2 | biostudies-other
ProteomicsCombinatorial native MS and LC-MS/MS approach reveals high intrinsic phosphorylation of human Tau
Abnormal changes in the neuronal microtubule-associated protein Tau, such as high phosphorylation and aggregation, are considered hallmarks of cognitive deficits in Alzheimer disease. Abnormal phosphorylation is thought to take place before aggregation, and therefore it is often assumed that phospho...
ORGANISM(S): Spodoptera frugiperda Homo sapiens (Human) Escherichia coli 
2020-11-20 | PXD020985 | Pride
Alzheimer disease Native mass spectrometry Tau protein Phosphorylation Liquid chromatography-mass spectrometry 
Proteomicsp53-regulated networks of protein, mRNA, miRNA and lncRNA expression revealed by integrated pSILAC and NGS analyses
We determined the effect of p53 activation on de novo protein synthesis using quantitative proteomics of newly synthesized proteins (pulsed stable isotope labeling with amino acids in cell culture, pSILAC) in combination with mRNA and non-coding RNA expression analyses by next generation sequencing ...
ORGANISM(S): Homo sapiens (Human) 
2015-07-23 | PXD001976 | Pride
Psilac Human Transcriptional network Biomarker P53 Tumor-suppressor 
ProteomicsIdentification of novel STAT6-regulated proteins in mouse B cells by comparative transcriptome and proteome analysis
IL-4/STAT6-regulated transcriptome and proteome were compared in primary B cells isolated from wild-type and STAT6-deficient mice. B cells were purified from the spleen and stimulated in vitro with anti-CD40 and LPS or anti-IgM-F(ab)2 in the presence or absence of IL-4. Transcriptome analysis was pe...
ORGANISM(S): Mus musculus (Mouse) 
2017-03-06 | PXD005224 | Pride
Stat6 Label-free quantitative mass spectrometry B cells Il-4 
ProteomicsThe universally conserved ATPase YchF regulates translation of leaderless mRNA in response to stress conditions
The universally conserved P-loop GTPases control diverse cellular processes, like signal transduction, ribosome assembly, cell motility, intracellular transport and translation. YchF belongs to the Obg-family of P-loop GTPases and is one of the least characterized member of this family. It is uniqu...
ORGANISM(S): Escherichia coli 
2021-09-09 | PXD023192 | Pride
Site-directed in vivo photo cross-linking Bacteria Lc-ms/ms 
ProteomicsInvolvement of RNase J in CRISPR RNA maturation and degradosome formation in cyanobacteria
Many bacteria and archaea use CRISPR-Cas systems, which provide RNA-based, adaptive, and inheritable immune defenses against invading viruses and other foreign genetic elements. The proper processing of CRISPR guide RNAs (crRNAs) is a crucial step in the maturation of the defense complexes and is fr...
ORGANISM(S): Synechocystis sp. PCC 6803 
2025-10-06 | PXD060866 | Pride
Cyanobacteria Degradosome Ribonuclease j Rna structure Rna:rna interaction Gene expression regulation Crispr-cas systems 
ProteomicsThe SecYEG interactome of E. coli: non-competitive binding of PpiD and YidC to the SecYEG translocon
The SecYEG translocon constitutes the major protein transporting channel in bacteria and transports an enormous variety of different secretory and inner membrane proteins. The minimal core of the SecYEG translocon consists of the three inner membrane proteins SecY, SecE and SecG, which together with...
ORGANISM(S): Escherichia coli 
2019-11-07 | PXD015974 | Pride
Yidc Ppid Label-free proteomics Secyeg translocon Protein cross-linking 
ProteomicsNon-competitive binding of PpiD and YidC to the SecYEG translocon expands the global view on the SecYEG interactome in E. coli
The SecYEG translocon constitutes the major protein transport channel in bacteria and transfers an enormous variety of different secretory and inner-membrane proteins. The minimal core of the SecYEG translocon consists of three inner-membrane proteins, SecY, SecE, and SecG, which, together with appr...
ORGANISM(S): Escherichia coli 
2019-11-07 | PXD015985 | Pride
Interaction proteomics Yidc Ppid Protein translocation Secyeg translocon Protein cross-linking Protein-protein interaction Quantitative affinity purification mass spectrometry (qap-ms) 
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