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ProteomicsPulldown with DNA mimicking foldamers in preblastoderm Drosophila embryo extract
16mer and 32mer biotinylated DNA mimics (“foldamers”) bound to streptavidin beads, AP-MS after 1h incubation in Drosophila preblastoderm embryo extract.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-09-07 | PXD040157 | Pride
Dna mimcs Lc-ms Exploris 480 In vitro chromatin assembly Drosophila embryo extract (drex) 
ProteomicsDrosophila embryo extract assisted in vitro chromatin assembly in presence of foldamer
Drosophila preblastoderm embryo extract assisted chromatin assembly with or without 16mer DNA mimic. Pull down of chromatin fiber to reveal chromatin binders in the system.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-09-07 | PXD039966 | Pride
Dna mimcs Qexactive hf Lc-ms In vitro chromatin assembly Drosophila embryo extract (drex) 
ProteomicsDNA mimicking 32mer foldamers interfere with in vitro chromatin assembly in drosophila embryo extract
Systematic study of the influence of different concentrations of DNA-mimicking foldamers (32mer) on the chromatin-bound bound proteome using an in vitro chromatin assembly extract
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-09-07 | PXD040158 | Pride
Dna mimcs Lc-ms Exploris 480 In vitro chromatin assembly Drosophila embryo extract (drex) 
ProteomicsSensing and signalling of DNA breaks in extracts of preblastoderm Drosophila embryos
Preblastoderm Drosophila embryos replicate their genomes at maximal speed. The assembly of chromatin on the newly synthesized chromosomes relies entirely on high concentrations of maternal nucleosome assembly and remodelling factors. Extracts from preblastoderm embryos can be turned into a system fo...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-10-14 | PXD013130 | Pride
Dna repair Dna damage response D.melanogaster 
ProteomicsIn vitro chromatin assembly - A quantitative proteomic analysis of in vitro assembled chromatin
Here, we use LC-MSMS and SWATH-MS to describe the kinetics of in vitro assembled chromatin supported by an embryo extract prepared from preblastoderm Drosophila melanogaster embryos. This system allows easy manipulation of distinct aspects of chromatin assembly such as post-translational histone mod...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2016-01-27 | PXD003445 | Pride
Lc-msms Chromatin assembly Drosophila melanogaster 
TranscriptomicsBinding site analysis and identification of reciprocal targeting of Dom-A and Xbp1 in Drosophila melanogaster
The DOM-A complex regulates cell growth and proliferation in Drosophila. Analogous to the mammalian Tip60–p400 complex, DOM-A integrates two epigenetic effectors: a SWR1-type ATPase (Domino-A) that mediates histone exchange and the Tip60/KAT5 acetyltransferase. We identified Xbp1, a conserved transc...
ORGANISM(S): Drosophila melanogaster 
2025-10-20 | E-MTAB-15796 | biostudies-arrayexpress
ProteomicsA quantitative proteomic analysis of in vitro assembled chromatin
The structure of chromatin is critical for many aspects of cellular physiology and is considered to be the primary medium to store epigenetic information. It is defined by the histone molecules that constitute the nucleosome, the positioning of the nucleosomes along the DNA and the non-histone prote...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2016-01-27 | PXD002537 | Pride
Acetylation  affinity proteomics  chromatin function or biology  label-free quantification   macromolecular assemblages 
ProteomicsIdentification of DNA-damage sensing factors in D.melanogaster
In order to identify protein involved in sensing DNA double strand breaks and recruiting the repair machinry, we performed in vitro assembly of chromatin on small circular and linear DNA templates, respectively. Proteins involved in chromatin assembly and sensing of free DNA ends were isolated and s...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-10-14 | PXD012990 | Pride
Dna double strand break Repair factors Chromatin assembly 
UnknownData on the kinetics of in vitro assembled chromatin.
Not available
S-EPMC4908269 | biostudies-literature
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