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Huh7 cells with or without expression of Flag-INSIG1/2 were treated with or without high glucose for 2 h. An immunoprecipitation assay was performed using anti-Flag antibody, and immunoprecipitates of Flag-INSIG1 were eluted with Flag peptide, separated using SDS-PAGE and stained with Coomassie Bril...
ORGANISM(S): Homo sapiens (Human) 
2025-12-26 | PXD054753 | Pride
Following high-glucose treatment of approximately 2 × 10⁸ Huh7 cells, immunoprecipitation was performed using an anti-INSIG1 antibody. Subsequent mass spectrometric analysis of a tryptic fragment identified succination at C167.
ORGANISM(S): Homo sapiens (Human) 
2025-12-26 | PXD071572 | Pride
The lem mutant was discovered in a doubled haploid (DH) line derived from the F1 of Gui-630 (Indica)xTaiwanjing (Japonica) by anther culture. The lem mutant did not affect other traits but caused all other floral organs (including palea, lodicule, stamen and carpel) to homeotically transform into le...
ORGANISM(S): Oryza sativa Indica Group 
Hypoxia represents a major physiological challenge for prawns and is a problem in aquaculture. Therefore, an understanding of the metabolic response mechanism of economically important prawn species to hypoxia and re-oxygenation is essential. However, little is known about the intrinsic mechanisms b...
2021-05-17 | MTBLS584 | MetaboLights
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