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ProteomicsTime-course proteomic analysis of spontaneously differentiating human embryonic stem cells (hESCs)
Mass spectrometry analysis was carried out to investigate the protein expression landscape of RNA-binding proteins (RBPs) during a time-course of spontaneously differentiating human embryonic stem cells (hESCs). Mass spectrometry was performed on whole-cell extracts of differentiating TE03 (I3) hESC...
ORGANISM(S): Homo sapiens (Human) 
2023-05-05 | PXD025274 | Pride
Early differentiation Spontaneously differentiating Shotgun proteomics Hescs Mass spectrometry Rbps Rna-binding proteins Human embryonic stem cells 
ProteomicsMass spectrometry based-proteomic analysis comparing the abundance of RNA-binding proteins (RBPs) between TE03 (I3) human embryonic stem cells (hESCs) and human foreskin fibroblasts (HFFs)
Here, we used high-resolution mass spectrometry to identify differentially expressed RNA-binding proteins (RBPs) between TE03 (I3) human embryonic stem cells (hESCs) and human foreskin fibroblasts (HFFs). 2102Ep embryonal carcinoma (EC) cells were used as a reference for pluripotent cells. This data...
ORGANISM(S): Homo sapiens (Human) 
2023-05-05 | PXD025284 | Pride
Shotgun proteomics Hescs Mass spectrometry Human foreskin fibroblasts Rbps Rna-binding proteins Human embryonic stem cells Hffs 
ProteomicsRNA-interactome capture in human embryonic stem cells (hESCs)
Embryonic stem cell (ESC) self-renewal and cell-fate decisions are driven by a broad array of molecular signals. While transcriptional regulators have been extensively studied in human ESCs (hESCs), the extent to which RNA-binding proteins (RBPs) contribute to human pluripotency remains unclear. Her...
ORGANISM(S): Homo sapiens (Human) 
2023-05-05 | PXD025194 | Pride
Rna-interactome capture Ric Hescs Rna-binding proeins Rbps Human embryonic stem cells 
ProteomicsThe nuclear RNA-interactome of human embryonic stem cells (hESCs)
Here, we performed RNA-interactome capture (RIC) on nuclear fractions from human embryonic stem cells (hESCs). The poly(A)+ RNA-bound proteome was determined by UV light-mediated cross-linking (CL) of RNAs to proteins in living cells, followed by nuclei isolation, oligo(dT) purification of poly(A)-R...
ORGANISM(S): Homo sapiens (Human) 
2023-05-05 | PXD025262 | Pride
Nuclear rna interactome Ric Hescs Rna-binding proteome Mass spectrometry Rbps Rna-binding proteins Rbpome Human embryonic stem cells Rna-interactome capture 
ProteomicsDeep proteomic profiling of TE03 (I3) human embryonic stem cells (hESCs)
The proteome of undifferentiated human embryonic stem cells (hESCs) was profiled by deep mass spectrometry-based proteomics of whole-cell extracts from suspension cultures of TE03 cells, in four biological replicates. This data accompanies the manuscript: "Uncovering the RNA-binding protein landscap...
ORGANISM(S): Homo sapiens (Human) 
2023-05-05 | PXD025278 | Pride
Hescs Human embryonic stem cells Deep proteomics 
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