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ProteomicsNAE1/UBA3-UBE2M are E1 and E2 Enzymes for the URM1 Modification in Human Cells
The Urm1 protein, a member of the ubiquitin (Ub)-related modifier family, is highly conserved across evolution and plays a crucial role as a sulfur donor in tRNA thiolation. Despite its importance, Urm1 remains relatively understudied in mammalian eukaryotes, leading to numerous unknown functions...
ORGANISM(S): Homo sapiens (Human) 
2026-03-23 | PXD067405 | Pride
E1/e2 enzymes lc-ms/ms 
ProteomicsInhibition of Ubiquitin Thioester Transfer by Targeting a Helix-in-Groove Interaction between E1 and E2
In eukaryotes, E1 initiates the ubiquitin cascade by adenylation and thioesterification of the ubiquitin C-terminus and subsequent transfer of ubiquitin to E2 enzymes. A clinical-grade small molecule that binds to the E1 ATP binding site and covalently derivatizes the ubiquitin C-terminus effectivel...
ORGANISM(S): Homo sapiens (Human) 
2020-08-18 | PXD016014 | Pride
Protein degradation Hdxms Conformational change Hydrogen deuterium exchange Ubiquitin ligase 
ProteomicsMapping ISG15 sites on GAPDH and PGK1 by AP-MS
We employed an AP-MS approach to map ISG15 modification sites on GAPDH and PGK1 in HEK293T cells by overexpression of FLAG-GAPDH, FLAG-PGK1 or FLAG-GFP together with the ISG15 conjugation enzymes (E1, E2, E3) and wild-type HA-ISG15 or conjugation-defective ISG15-AA. Cells were lysed in physiological...
ORGANISM(S): Homo sapiens (Human) 
2026-03-02 | PXD071724 | Pride
Ap-ms Pgk1 Gapdh Isg15 Glycolysis 
ProteomicsLC-MS/MS analysis of ribosome UFMylation in vitro
UFMylation, is a Ubiquitin-like modification occurring on RPL26, a ribosomal subunit, upon ribosome stalling at the ER membrane. This modification is achieved by a pool of enzymes commonly referred to as E1-activating, E2-conjugating and E3-ligase enzymes. The UFM1 E3 ligase comprises of three prote...
ORGANISM(S): Homo sapiens (Human) 
2024-03-22 | PXD046991 | Pride
In vitro reconstitution Rpl26 ufmylation Ribosome ufmylation 
MetabolomicsProteomics and tracer metabolomics link GAPDH ISGylation to glycolytic control

Ubiquitin-like protein ISG15 (interferon-stimulated gene 15) has been implicated in the regulation of central carbon metabolism, but conflicting findings across experimental systems have limited mechanistic insight. Here, we applied a multi-omics approach in cells ectopically expressing the ISGyl...

2026-03-02 | MTBLS12619 | MetaboLights
MetabolomicsFumarate reprograms the ubiquitinated proteome to confer ferroptosis resistance in clear cell renal cell carcinoma

Clear cell renal cell carcinoma (ccRCC) is a kidney malignancy characterized by aberrant lipid accumulation and is intrinsically sensitive to ferroptosis. However, the mechanisms by which ccRCC escapes ferroptotic vulnerability remain poorly understood. Here, we report that fumarate hydratase (FH...

2026-10-16 | MTBLS15364 | MetaboLights
ProteomicsOrthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin enzymes
Protein ubiquitination is mediated sequentially by ubiquitin activating enzyme E1, ubiquitin conjugating enzyme E2, and ubiquitin ligase E3. Uba1 was thought to be the only E1 until the recent identification of Uba6 as an alternative. To differentiate the biological functions of Uba1 and Uba6, we ap...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD005500 | Pride
Lc/ms Ubquitin 
ProteomicsAuranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterificlation to E2 ubiquitin-conjugating enzymes
UBA1 is the primary E1 ubiquitin-activating enzyme, regulating stability and function of numerous proteins via initiating their ubiquitination. Decreased or insufficient ubiquitination can cause or drive aging and many deadly diseases. Therefore, a small-molecule UBA1 activity enhancer could have br...
ORGANISM(S): Homo sapiens (Human) 
2023-07-10 | PXD042558 | Pride
Ubiquitination Uba1 Auranofin 
UnknownThe FAT10 post-translational modification is involved in the lytic replication of Kaposi's sarcoma-associated herpesvirus.
Not available
S-EPMC8139669 | biostudies-literature
ProteomicsIdentification by activity-based probes of a new Plasmodium falciparum E2 ubiquitin conjugating enzyme
The ubiquitin-proteasome system (UPS) is essential for Plasmodium falciparum survival and represents a potential target for antimalarial therapies. We utilized a ubiquitin-based activity probe (Ub-Dha) to capture active ubiquitin conjugating machinery during asexual blood-stage development. Several ...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2025-03-13 | PXD056473 | Pride
Plasmodium falciparum Activity proteomics Ubiquitin 
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