Sort   by:  
 Page size 
MetabolomicsNon-targeted in vitro metabolomics for proteome-scale identification of novel enzymes in Escherichia coli
Many yet undiscovered enzymes still limit our understanding of metabolism. Here, we develop a high-throughput mass spectrometry approach to comprehensively profile proteome-scale protein collections for in vitro enzymatic activity. Overexpressed or purified proteins are incubated in a supplemented m...
2016-01-11 | MTBLS373 | MetaboLights
MetabolomicsCalenduloside E produced by Bifidobacterium animalis B960 increases glibenclamide efficacy and alleviates drug-induced adverse effects via the IRS1/PI3K/AKT signaling pathway in a type 2 diabetes model Calenduloside E produced by Bifidobacterium animalis B960 increases glibenclamide efficacy and alleviates drug-induced adverse effects via the IRS1/PI3K/AKT signaling pathway in cell, organ-on-a-chip, and mouse models of type 2 diabetes.

Glibenclamide (GLIB), a type 2 diabetes therapy, has long-term safety concerns. Probiotic–drug coadministration, an emerging adjunctive approach for metabolic diseases, improves therapeutic outcomes and side effects. Here, Bifidobacterium animalis B960 was selected from 71 probiotic strains by HP...

2025-07-08 | MTBLS12697 | MetaboLights
ProteomicsThe human cholesterogenic enzymes DHCR14 and LBR are differentially regulated transcriptionally and post-translationally
Cholesterol synthesis is a tightly regulated process, both transcriptionally and post-translationally. Transcriptional control of cholesterol synthesis is relatively well understood. However, of the ~20 enzymes in cholesterol biosynthesis, post-translational regulation has only been examined for a s...
ORGANISM(S): Homo sapiens (Human) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-01-15 | PXD016417 | Pride
Dhcr14 Lbr Cholesterol synthesis enzymes 
ProteomicsTULIP2: an improved method for the identification of ubiquitin E3-specific targets
Protein modification by Ubiquitin or Ubiquitin-like modifiers is mediated by an enzyme cascade composed of E1s, E2s and E3s enzymes. E1, or ubiquitin-activating enzymes, perform the ubiquitin activation. Next, ubiquitin is transferred to ubiquitin-conjugating enzymes or E2s. Finally, ubiquitin ligas...
ORGANISM(S): Homo sapiens (Human) 
2019-11-15 | PXD015437 | Pride
Ubiquitin E3 enzymes Mass spectrometry Post-translational modifications Proteomics 
UnknownA cascading activity-based probe sequentially targets E1-E2-E3 ubiquitin enzymes.
Not available
S-EPMC5108872 | biostudies-literature
Proteomics1A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transporters
Cells rapidly remodel their proteomes to align their cellular metabolism to environmentalconditions. Ubiquitin E3 ligases enablethis response, by facilitatingrapid andreversible changes to protein stability, localization, or interaction partners. In S. cerevisiae, the GID E3 ligase ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-03-02 | PXD030902 | Pride
Gid Proteomics Nutrient signaling Dia Ms Ubiquitin e3 ligase Metabolism Rsp5 
ProteomicsCovalent fragment screening to inhibit the E3 ligase activity of bacterial NEL enzymes SspH1 and SspH2
As the global fight against antimicrobial resistance in bacteria becomes increasingly pressing, new tool compounds are needed to understand and identify novel therapeutic targets. Here, cysteine-directed fragment-based drug discovery is coupled with a high throughput chemistry direct-to-biology scre...
ORGANISM(S): Homo sapiens (Human) 
2025-11-10 | PXD057304 | Pride
Bacterial nels Fragment-based drug discovery Covalent fragments Salmonella effectors Chemoproteomics E3 ligase High throughput chemistry 
TranscriptomicsE2-RING expansion of the NEDD8 cascade confers specificity to cullin modification
Ubiquitin and ubiquitin-like proteins (UBLs) are directed to targets by cascades of E1, E2, and E3 enzymes. The largest ubiquitin E3 subclass consists of cullin-RING ligases (CRLs), which contain one each of several cullins (CUL1, -2, -3, -4, or -5) and RING proteins (RBX1 or -2). CRLs are activated...
ORGANISM(S): Mus musculus 
2009-03-23 | E-GEOD-14088 | biostudies-arrayexpress
ProteomicsMulti-scale classification decodes the complexity of the human E3 ligome
E3 ubiquitin ligases are key regulators of protein homeostasis, targeting specific proteins for degradation via the ubiquitin-proteasome system (UPS). They provide crucial substrate specificity, making them promising candidates for the design of novel therapeutics. This work presents a comprehensive...
ORGANISM(S): Homo sapiens (Human) 
2025-11-18 | PXD067015 | Pride
E3 ligase 
UnknownMulti-tiered pairing selectivity between E2 ubiquitin-conjugating enzymes and E3 ligases.
Not available
S-EPMC6200922 | biostudies-literature
Sort   by:  
 Page size