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ProteomicseIF4A3 interactome by IP-MS in mouse ESCs
RNA helicases are involved in multiple steps of RNA metabolism to direct their roles in gene expression, yet their functions in pluripotency control remain largely unexplored. Starting from an RNAi screen of RNA helicases, we identified that eIF4A3, a DEAD-box (Ddx) helicase component of the exon ju...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-11 | PXD031362 | Pride
Ip-ms Eif4a3 Escs 
TranscriptomicsRNA-Seq of control and EJC factor (EIF4A3, RBM8A, MAGOH) knockdowns in part with EIF4A3 rescue in HeLa Tet-Off cells
The exon junction complex is involved in gene expression regulation on multiple co- and post-transcriptional levels. We aimed to investigate in HeLa Tet-Off cells which gene expression alterations can be observed upon knockdown of core EJC factors (EIF4A3, RBM8A and MAGOH) or upon EIF4A3 knockdown a...
ORGANISM(S): Homo sapiens 
2022-05-06 | E-MTAB-10771 | biostudies-arrayexpress
TranscriptomicsPRPF8 iCLIP on HeLa cells in response to eIF4A3 Knockdown
This experiment uses iCLIP to identify the binding pattern of the spliceosomal protein PRPF8 on RNA. The data shows that PRPF8 binds strongly and specifically in the region 12 to 14nt upstream of 5' splice sites (5ss). Due to PRPF8's role in the formation of the catalytically active spliceosome, thi...
ORGANISM(S): Homo sapiens 
2018-10-14 | E-MTAB-7269 | biostudies-arrayexpress
ProteomicsU2OS LC MSMS upon siRNA-eIF4A3 or 5nM ActD treatment.
Proteome analysis in U2OS cells treated with siRNA against eIf4A3 or 5nM Actinomycin D.
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD016005 | Pride
U2os lc msms upon sirna-eif4a3 or 5nm actd treatment. 
TranscriptomicsRNA-Seq of EIF4A3 knockdown in human embryonic kidney cell line HEK293 via siRNA
EIF4A3 is one of the three core components of the exon junction complex (EJC). The EJC is deposited during pre-mRNA splicing upstream of the exon-exon junction and serves as a mark for exon boundaries. The EJC is involved in various gene expression regulatory mechanisms, such as nonsense-mediated de...
ORGANISM(S): Homo sapiens 
2026-09-16 | E-MTAB-13794 | biostudies-arrayexpress
GenomicseIF4A3 - Modified iCLIP
eIF4A3 - Modified iCLIP
PRJEB11647 | ENA
TranscriptomicsiCLIP of eIF4A3 and PTB in HeLa cells
HeLa cells were cultured in DMEM, supplemented with 10% (v/v) FCS and penicillin/streptomycin under 5% CO2 at 37°C. For iCLIP, HeLa cells expressing eIF4A3-GFP or PTB-GFP were induced with doxycycline to adjust the level of recombinant protein to the level of the endogenous counterpart and irradiate...
ORGANISM(S): Homo sapiens 
2015-08-11 | E-MTAB-2599 | biostudies-arrayexpress
GenomicsGenome-wide identification of circular RNAs in eIF4A3 IP
Genome-wide identification of circular RNAs in eIF4A3 IP
PRJNA1001827 | ENA
TranscriptomicseIF4A3 - Modified iCLIP
The modified iCLIP protocol is based on the previously described protocol (Huppertz et al., 2014) with modifications that enable the definition of readthrough cDNAs. HeLa cells were crosslinked with 0.15mJ/cm2 254nm UV light. Protein G Dynabeads were used for immunoprecipitations (IP). For each IP, ...
ORGANISM(S): Homo sapiens 
2016-11-24 | E-MTAB-4000 | biostudies-arrayexpress
GenomicsiCLIP of eIF4A3 and PTB in HeLa cells
iCLIP of eIF4A3 and PTB in HeLa cells
PRJEB6381 | ENA
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