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ProteomicsMass Spectrometry-Driven Epitope Mapping: Application of Diethylpyrocarbonate Covalent Labeling for the Immunotherapeutic Target Programmed Cell Death Protein 1
Monoclonal antibodies (mAbs) have transformed cancer immunotherapy, with anti-PD1 mAbs playing a pivotal role. This study introduces diethylpyrocarbonate covalent labeling-mass spectrometry (DEPC CL-MS) as a rapid and sensitive technique for epitope mapping of PD1, a critical target in mAb developme...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-11-26 | MSV000096536 | MassIVE
TranscriptomicsTranscription profiling of human VEGF-derived CD8+ T-cell epitope
The vascular endothelial growth factor A (VEGF) is involved in various physiological processes such as angiogenesis or wound healing but is also crucial in pathological events such as tumor growth. Thus, clinical anti-VEGF treatments have been developed which could already prove to have enormous ben...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-8050 | biostudies-arrayexpress
ProteomicsIntact Transition Epitope Mapping- Thermodynamic Weak-force Order (ITEM-TWO)
Most important characteristics of antibodies are that they typically strongly bind to specific epitopes, thereby expressing low dissociation constants (KDs) and high Gibbs free binding energies (delta Gs). In this study, we describe an off-line nano-electrospray mass spectrometry method, termed IT...
ORGANISM(S): Homo sapiens (Human) 
2019-11-07 | PXD016024 | Pride
Antibody affinity determination Antibody specificity determination 
TranscriptomicsGenome-wide chromatin binding analysis (ChIP-seq) of HA epitope tagged RFX6 protein in human embryonic stem cell derived pancreatic progenitors
To identify direct transcriptional targets of RFX6, we performed chromatin immunoprecipitation of HA epitope tagged RFX6 followed by massively parallel DNA sequencing (ChIP-seq). Using CRISPR/Cas9 gene editing, the HA epitope was inserted into the 3' end of the RFX6 gene in H9 hESC. Pluripotent cell...
ORGANISM(S): Homo sapiens 
2020-10-23 | E-MTAB-9335 | biostudies-arrayexpress
ModelsSanjuan2013 - Evolution of HIV T-cell epitope, control model
Sanjuan2013 - Evolution of HIV T-cell epitope, control model Control model in which the virus targets a nonimmune cell type C (e.g., hepatocytes, epithelial cells, etc.), instead of T H cells. This model is described in the article:...
2005-01-01 | MODEL1302180002 | BioModels
ProteomicsMulti-epitope immunocapture of huntingtin reveals striatum-selective molecular signatures in Huntington's disease mouse models
Huntington's disease (HD) is a debilitating progressive neurodegenerative disorder that has profound effects on an individual's cognitive, motor, and behavioral functions. HD is caused by mutation in the huntingtin (HTT) gene that results in CAG repeat expansion and production of an aggregation-pron...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2024-04-10 | MSV000094509 | MassIVE
ModelsSanjuan2013 - Evolution of HIV T-cell epitope, immune activation model
Sanjuan2013 - Evolution of HIV T-cell epitope, immune activation model Model of cellular immune response against HIV. This model is described in the article: Immune activation promotes evolutionary conservation of T-cell epitopes ...
2005-01-01 | MODEL1302180001 | BioModels
ProteomicsIntact Transition Epitope Mapping-Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE)
ITEM-THREE is a novel mass spectrometry-based epitope mapping method. To perform ITEM-THREE, antibody-epitope peptide immune-complexes are first generated in electrospray-compatible solutions in which the antibodies maintain their in-solution functionality, by adding an antibody of interest to a mi...
ORGANISM(S): Homo sapiens (Human) 
2019-06-03 | PXD013823 | Pride
Human Molecular recognition Nanoesi Recombinant proteins Ion mobility separation Item-three Collision induced dissociation Epitope antibody complexes 
ProteomicsThe Trypanosoma cruzi Antigen and Epitope Atlas: discovery of antibody specificities in human Chagas Disease patients across human populations
These assays represent an antigen discovery screening, and epitope mapping characterization. In this screening two complete proteomes from Trypanosoma cruzi, from two different strains (CL-Brener, Sylvio X10), were displayed in the form of short peptides (tiling array, overlapped) and assayed with p...
ORGANISM(S): Homo sapiens 
2022-08-20 | E-MTAB-11651 | biostudies-arrayexpress
ProteomicsThe Trypanosoma cruzi Antigen and Epitope Atlas: fine characterization of antibody specificities in human Chagas Disease patients across human populations
These assays represent antibody-binding assays to analyze seroprevalence of antigens and epitopes at the individual level and also to perform fine epitope mapping characterizations. In this screening 392,299 peptides derived from reactive (antigenic) regions of Trypanosoma cruzi proteins were displa...
ORGANISM(S): Homo sapiens 
2022-08-20 | E-MTAB-11655 | biostudies-arrayexpress
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