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ProteomicsErk quantitative phosphorylation
To identify downstream phosphorylation substrates of Erk, we treated the iErk1; Erk KO cell line with and without doxycycline (DOX) for 48 hours. In the untreated group, intracellular Erk was found to be deficient. Mass spectrometry-based quantitative analysis was subsequently used to identify phosp...
ORGANISM(S): Mus musculus (Mouse) 
2025-01-07 | PXD051508 | Pride
Mouse Erk 
TranscriptomicsTranscriptional Profiling Defines the Roles of ERK and p38 Kinases in Epidermal Keratinocytes
Epidermal keratinocytes respond to extracellular influences by activating cytoplasmic signal transduction pathways that change the transcriptional profiles of affected cells. To define responses to two such pathways, p38 and ERK, we used SB203580 and PD98059 as specific inhibitors, and identified th...
ORGANISM(S): Homo sapiens 
2013-09-05 | E-GEOD-50591 | biostudies-arrayexpress
ProteomicsContext-dependent regulatory mechanisms control site-specific information processing within the MEK/ERK module
The impact of the cellular context and receptor type on the dynamics of phosphorylation cycles in signaling pathways are unclear. We employ an unbiased approach to identify network alterations that explain phosphorylation dynamics of the MEK/ERK module in response to hepatocyte growth factor or inte...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080803 | MassIVE
TranscriptomicsRNA-seq of canine MDCK epithelial cell line compared to cell line overexpressing ERK2 or cell line with conditional activation of the ERK pathway
Using RNA-seq and canine MDCK epithelial cells, we analyzed mRNA expression profiles of cells overexpressing ERK2 and cells where the ERK pathway was activated for 24 hrs. For this purpose, three cell lines were used: parental MDCK cells, MDCK cells overexpressing FLAG-ERK2 or MDCK cells expressing ...
ORGANISM(S): Canis lupus familiaris 
2022-10-01 | E-MTAB-12132 | biostudies-arrayexpress
ProteomicsProtein abundance of AKT and ERK pathway components governs cell-type-specific regulation of proliferation
Signaling through the AKT and ERK pathways controls cell proliferation. However, the integrated regulation of this multistep process, involving signal processing, cell growth and cell-cycle progression, is poorly understood. Here we study different murine hematopoietic cell types, in which AKT and E...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080844 | MassIVE
TranscriptomicsCritical requirement of ERK/MAPK signaling in respiratory tract development
The mammalian genome contains two ERK/MAP kinase kinase genes, Mek1 and Mek2, which encode dual-specificity kinases responsible for ERK/MAP kinase activation. To define the function of ERK/MAPK signaling pathway in lung development, we performed tissue-specific deletions of Mek1 function in a Mek...
ORGANISM(S): Mus musculus 
2014-09-02 | E-GEOD-51643 | biostudies-arrayexpress
TranscriptomicsAberrant ERK signaling causes resistance to EGFR kinase inhibitors
The clinical efficacy of EGFR kinase inhibitors is limited by the development of drug resistance. The irreversible EGFR kinase inhibitor WZ4002 is effective against the most common mechanism of drug resistance mediated by the EGFR T790M mutation. Here we show that in multiple complementary models ha...
ORGANISM(S): Homo sapiens 
2012-10-01 | E-GEOD-37699 | biostudies-arrayexpress
TranscriptomicsERK Oscillation-Dependent Gene Expression Patterns and Deregulation By The Stress-Response
Studies were undertaken to determine whether oscillatory behavior in the extracellular signal regulated kinase (ERK) pathway results in unique gene regulation patterns. Microarray analysis was performed on three subcloned populations of human keratinocytes with distinct ERK signaling/oscillation ph...
ORGANISM(S): Homo sapiens 
2014-03-13 | E-GEOD-55823 | biostudies-arrayexpress
TranscriptomicsETS1 is a genome-wide effector of RAS/ERK signaling in epithelial cells (RNA-Seq)
ETS1 and RAS/ERK regulate a common gene expression program in establishing enviroment suitable for prostate cancer cell migration. mRNA profiles of luciferase knockdown (WT), ETS1 knockdown, and U0126 treated DU145 cells were generated using deep sequencing, in triplicate, using Illumina HiSeq. Knoc...
ORGANISM(S): Homo sapiens 
2014-10-07 | E-GEOD-59020 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse NIH3T3 cell line cultured with and withput FGF and treated with the MEK Inhibitor U0126 at 3 time points before FGF addition reveals ERK-dependent downregulated genes have the ability to block S phase entry
The ERK family of MAP kinase plays a critical role in growth factor-stimulated cell cycle progression from G0/G1 to S phase. But, how sustained activation of ERK promotes G1 progression has remained unclear. Here, our systematic analysis on the temporal program of ERK-dependent gene expression shows...
ORGANISM(S): Mus musculus 
2007-11-24 | E-GEOD-4739 | biostudies-arrayexpress
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