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TranscriptomicsEffect of EWS/FLI1 and DAX1 silencing on the gene expression profile of the A673 Ewing cell line
The molecular hallmark of the Ewing family of tumors is the presence of balanced chromosomal translocations leading to the formation of chimerical transcription factors (i.e. EWS/FLI1) that play a pivotal role in the pathogenesis of Ewing tumors by deregulating gene expression. We have recently demo...
ORGANISM(S): Homo sapiens 
2012-02-23 | E-GEOD-36007 | biostudies-arrayexpress
ProteomicsUSP1 expression driven by EWS-FLI1 transcription factor stabilizes Survivin and promotes Ewing sarcoma cell survival
Ewing sarcoma (EWS) is a malignant pediatric bone cancer. Most Ewing sarcomas are driven by EWS-FLI1 oncogenic transcription factor that plays roles in transcriptional regulation, DNA damage response, cell cycle checkpoint control, and alternative splicing. USP1, a deubiquitylase which regulates DNA...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD041033 | Pride
Ews-fli1 Usp1 Survivin 
TranscriptomicsOncogenic Fusion Protein EWS-FLI1 is a Network Hub that Regulates Alternative Splicing
The synthesis and processing of mRNA, from transcription to translation initiation, often requires splicing of intragenic material. The final mRNA composition varies based upon proteins that modulate splice site selection. EWS-FLI1 is an Ewing sarcoma (ES) oncogene with an interactome that we demons...
ORGANISM(S): Homo sapiens 
2015-03-31 | E-GEOD-65941 | biostudies-arrayexpress
GenomicsTumor-specific retargeting of an oncogenic transcription factor chimera results in dysregulation of chromatin and transcription
Using EWS-FLI and its parental transcription factor, FLI1, we created a unique experimental system to address questions regarding the genomic mechanisms by which chimeric transcription factors cause cancer. We found that in tumor cells, EWS-FLI targets regions of the genome distinct from FLI1, despi...
ORGANISM(S): Homo sapiens 
2011-11-09 | E-GEOD-31838 | biostudies-arrayexpress
TranscriptomicsA zebrafish transgenic model of Ewing's Sarcoma reveals conserved mediators of EWS-FLI1 tumorigenesis
This SuperSeries is composed of the following subset Series: GSE31185: The human Ewing's Sarcoma oncoprotein EWS-FLI1 causes developmental defects in zebrafish embryos GSE31186: The human Ewing's Sarcoma oncoprotein EWS-FLI1 causes Ewing's-type tumors in zebrafish Refer to individual Series
ORGANISM(S): Danio rerio 
2011-11-01 | E-GEOD-31217 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human HEK293 cells transfected with EWS-FLI1 (Ewing sarcoma family fusion gene)
Response of HEK293-cells after transfection with EWS-FLI1. HEK293 cells were transfected with the expression vector pIRES2-EGFP containing type I EWS-FLI1 or empty control vector. For transient transfection standard DEAE dextran method was used and RNA was isolated 48h post transfection. For stable ...
ORGANISM(S): Homo sapiens 
2007-08-25 | E-GEOD-1822 | biostudies-arrayexpress
TranscriptomicsThe human Ewing's Sarcoma oncoprotein EWS-FLI1 causes Ewing's-type tumors in zebrafish
The fusion oncoprotein EWS-FLI1 arises from a t(11;22)(q24;q12) chromosomal translocation and causes Ewing's Sarcoma, a malignant bone tumor. The mechanism whereby EWS-FLI1 transforms cells is unknown. Somatic, mosaic expression of human EWS-FLI1 in zebrafish from the heat shock promoter [Tg(HSP:...
ORGANISM(S): Danio rerio 
2011-11-01 | E-GEOD-31186 | biostudies-arrayexpress
TranscriptomicsLow-dose actinomycin D preferentially disrupts EWS-FLI1DNA binding.
Fusion of the EWS gene to FLI1 produces a fusion oncoprotein that drives an aberrant gene expression program responsible for the development of Ewing sarcoma. We used a homogenous proximity assay to screen for compounds that disrupt the binding of EWS-FLI1 to its cognate DNA targets. A number of DNA...
ORGANISM(S): Homo sapiens 
2013-08-26 | E-GEOD-45414 | biostudies-arrayexpress
TranscriptomicsThe human Ewing's Sarcoma oncoprotein EWS-FLI1 causes developmental defects in zebrafish embryos
The fusion oncoprotein EWS-FLI1 arises from a t(11;22)(q24;q12) chromosomal translocation and causes Ewing's Sarcoma, a malignant bone tumor. The mechanism whereby EWS-FLI1 transforms cells is unknown. We made germline transgenic zebrafish expressing human EWS-FLI1 under the control of the heat s...
ORGANISM(S): Danio rerio 
2011-11-01 | E-GEOD-31185 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human Ewing sarcoma samples and EWS-FLI-1 inhibited Ewing sarcoma cell lines
The cellular origin of Ewing tumor (ET), a tumor of bone or soft tissues characterized by specific fusions between EWS and ETS genes, is highly debated. Through gene expression analysis comparing ETs with a variety of normal tissues, we show that the profiles of different EWS-FLI1-silenced Ewing cel...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7007 | biostudies-arrayexpress
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