Fachinetti Daniele D

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Transcriptomics (2)
Proteomics (2)

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Homo sapiens (2)
Schizophyllum commune H4-8 (2)
Mycobacterium tuberculosis SP7 (2)
Salmonella enterica subsp. enterica serovar Typhimurium str. T000240 (2)
Oryza sativa Japonica Group (2)
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Candidatus Endoriftia persephonae (2)
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Mycobacterium tuberculosis SP13 (2)
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Mycobacterium tuberculosis SP22 (2)
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Enterobacter ludwigii (2)
Trichoderma asperellum CBS 433.97 (2)
Corynebacterium diphtheriae bv. mitis str. ISS 3319 (2)
Nakaseomyces glabratus (2)
Olea europaea (2)
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Pyricularia oryzae P131 (2)
Enterococcus mundtii CRL35 (2)
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Oncorhynchus tshawytscha (2)
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biostudies-arrayexpress (2)
pride (2)

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Cell culture (2)
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Mass Spectrometry (2)
Affinity proteomics (1)
Chemical cross-linking coupled with mass spectrometry proteomics (1)
Shotgun proteomics (1)

Instrument Platform

Illumina NovaSeq 6000 (2)

Publication Date

2021 (2)
2024 (1)
2019 (1)

Study type

RNA-seq of coding RNA from single cells (1)
RNA-seq of coding RNA (1)

Release Date

2021 (2)

Lab affiliation

Institut Curie, PSL Research University, CNRS, UMR 144, 26 rue d’Ulm, F-75005, Paris, France. (1)
Institut Curie, CNRS, UMR 144, 26 rue dÃ¢â‚¬â„¢Ulm, 75005 Paris, France (1)

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Phosphorylation of CENP-A on serine 7 does not control centromere function

CENP-A is the histone H3 variant necessary to specify the location of all eukaryoticcentromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. On...

ORGANISM(S): Homo sapiens (Human)

2019-01-28 | PXD012163 | Pride

Specialized replication mechanisms maintain genome stability at human centromeres

Reveal a specific set of proteins important to maintain centromere integrity, through quantitative PICh (Proteomics of Isolated Chromatin). Centromeric chromatin was pulled down through RNA probes annealing specifically to a 300bp-long conserved centromeric sequence.

ORGANISM(S): Homo sapiens (Human)

2024-02-14 | PXD047847 | Pride

Replication stress Proteomics Genome instability Centromere Dna damage Dna replication

Bulk RNA-Seq to study the effects of CENP-A overexpression, p53 status, and X- irradiation treatment in MCF-10-2A cells

We performed bulk RNA-Seq to investigate global transcriptional changes upon overexpression of the centromeric H3 variant CenH3/CENP-A in p53 wild-type and defective cells, and after X-irradiation treatment. We established clonal MCF-10-2A TetOn-CENPA-FLAG-HA cell lines where CENP-A can be reversibl...

ORGANISM(S): Homo sapiens

2021-03-01 | E-MTAB-9867 | biostudies-arrayexpress

Single-cell RNA-Seq to study the effects of acute and chronic CENP-A overexpression depending on p53 status

We performed single-cell RNA-Seq to determine the effects of CENP-A overexpression and p53 status on cell fate over time. We established clonal MCF10-2A TetOn-CENPA-FLAG-HA cell lines where CENP-A can be reversibly induced by Doxycycline (Dox) treatment. We compared prolonged, continuous CENP-A over...