Transcription steps are marked by different modifications of the C-terminal domain of RNA polymerase II (RNAPII). Phosphorylation of Ser5 and Ser7 by cyclin-dependent kinase 7 (CDK7) as part of TFIIH marks initiation, whereas phosphorylation of Ser2 by CDK9 marks elongation. These processes are thou...
We have develop a simple fact method to isolate transcription factories and analyzed their RNA content as compared to total poly(A)+ and chromatin associated RNA fractions in presence or absence of pro-inflammatory cytokine.
The HSP90/R2TP quaternary chaperone assembles key cellular machines, including the three nuclear RNA polymerases and many non-coding RNPs. Here, we show that the R2TP binds many partners co-translationally. Remarkably, its co-translational interactome reveals many novel potential clients, and side-b...
Early in infection, mammalian orthoreoviruses (reoviruses) build neo-organelles called viral factories (VFs). These structures incorporate fragments of endoplasmic reticulum (ER) and serve as sites of viral genome replication and particle assembly. Two reovirus nonstructural proteins, σNS and µNS, r...
Global regulation of protein phosphorylation and dephosphorylation plays crucial roles in various cellular processes, serving as a fundamental mechanism for regulating protein function, cell metabolic performance and stress response. In response to differences in nutrition, alterations in the metabo...
