Sort   by:  
 Page size 
ProteomicsMulti-Enzymatic Limited Digestion - the Next-Generation Sequencing for Proteomics
An innovative approach merging both the bottom-up and middle-down ideologies to provide fast, robust and comprehensive solutions for proteomics studies. The methodology, referred to as MELD for Multiple-Enzymatic Limited Digestion, capitalizes on a single two-hour long proteolytic step involving the...
ORGANISM(S): Homo sapiens (Human) 
2019-05-08 | PXD009800 | Pride
Ptms Digestibility profile Limited digestion Mass spectrometry Proteolytic method Protein characterization De novo sequencing  mabs 
UnknownAntibody-drug conjugate model fast characterization by LC-MS following IdeS proteolytic digestion.
Not available
S-EPMC3929440 | biostudies-literature
GenomicsMicrofluidic enrichment of proteolytic microbial consortia from sewage sludge
Microfluidic enrichment reveals diverse proteolytic microorganisms compared to conventional screening
PRJEB107907 | ENA
UnknownCharacterization of Affitin proteolytic digestion in biorelevant media and improvement of their stabilities via protein engineering.
Not available
S-EPMC7661517 | biostudies-literature
ProteomicsBroad-specificity enzymes enable fast and cost-effective digestion for in-depth proteomics and precise label free quantitation
We have devised fast and robust SP3- and STRAP-based protocols for the broad-specificity proteases subtilisin, proteinase K, and thermolysin. All three enzymes are remarkably fast, producing near-complete digests in 1-5 min, and cost 200-1000x less than proteomics-grade trypsin. Using FragPipe resol...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-29 | MSV000093532 | MassIVE
ProteomicsChemical acetylation of ligands and two-step digestion protocol for reducing co-digestion in affinity purification-mass spectrometry
We present an effective, fast and user-friendly method to reduce co-digestion of bead-bound ligands, such as antibodies or streptavidin, in affinity purification-mass spectrometry experiments. A short pre-incubation of beads with Sulfo-NHS-Acetate leads to chemical acetylation of lysine residues, ma...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2023-10-23 | PXD043709 | Pride
Chemical acetylation Immunopurification Streptavidin Ligand Affinity purification Sulfo-nhs-acetate Nanobody Gfp 
ProteomicsShort enzymatic reactions in proteomics studies
SKBR3 breast cancer cell extracts were digested with trypsin (or LysC) on short time scales (7min, 15min, 30min, 1h, and 18h), and the results were compared to overnight digestion protocols.
ORGANISM(S): Homo sapiens (Human) 
2018-09-24 | PXD010306 | Pride
Proteomics Mass spectrometry Fast proteolytic digestion 
UnknownInter-laboratory study of an optimised peptide mapping workflow using automated trypsin digestion for monitoring monoclonal antibody product quality attributes.
Not available
S-EPMC7496030 | biostudies-literature
UnknownFacilitating protein disulfide mapping by a combination of pepsin digestion, electron transfer higher energy dissociation (EThcD), and a dedicated search algorithm SlinkS.
Not available
S-EPMC4189002 | biostudies-literature
UnknownComparison of Bloodmeal Digestion and the Peritrophic Matrix in Four Sand Fly Species Differing in Susceptibility to Leishmania donovani.
Not available
S-EPMC4452187 | biostudies-literature
Sort   by:  
 Page size