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ProteomicsChemical acetylation of ligands and two-step digestion protocol for reducing co-digestion in affinity purification-mass spectrometry
We present an effective, fast and user-friendly method to reduce co-digestion of bead-bound ligands, such as antibodies or streptavidin, in affinity purification-mass spectrometry experiments. A short pre-incubation of beads with Sulfo-NHS-Acetate leads to chemical acetylation of lysine residues, ma...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2023-10-23 | PXD043709 | Pride
Chemical acetylation Immunopurification Streptavidin Ligand Affinity purification Sulfo-nhs-acetate Nanobody Gfp 
UnknownChemical Acetylation of Ligands and Two-Step Digestion Protocol for Reducing Codigestion in Affinity Purification-Mass Spectrometry.
Not available
S-EPMC10563155 | biostudies-literature
ProteomicsChemical footprinting of Polyhomeotic-proximal1289-1577
The Polycomb protein Polyhomeotic (Ph) is implicated in organizing chromatin to regulate gene expression. This activity depends on the sterile alpha motif in Ph, which can form helical polymers. This polymerization activity is believed to create macromolecular assemblies of Polycomb proteins and chr...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2020-07-10 | MSV000085717 | MassIVE
UnknownSolution versus gas-phase modification of peptide cations with NHS-ester reagents.
Not available
S-EPMC3265610 | biostudies-literature
ProteomicsDirect Interactions of RNA Polymerase with Ribosomes and Ribosomal Subunits in E. Coli
The interactions between RNA polymerase and ribosomes are critical for the coordination of transcription with translation. We report that RNA polymerase directly binds ribosomes and isolated large and small ribosomal subunits.
ORGANISM(S): Escherichia coli 
2017-08-28 | PXD006717 | Pride
Ribosomes Mass spectrometry E. coli Rna polymerase 
UnknownThe LDL receptor-related protein 1 (LRP1) facilitates ACE2-mediated endocytosis of SARS-CoV2 spike protein-containing pseudovirions.
Not available
S-EPMC12192683 | biostudies-literature
UnknownGas-Phase Ion/Ion Chemistry for Structurally Sensitive Probes of Gaseous Protein Ion Structure: Electrostatic and Electrostatic to Covalent Cross-Linking.
Not available
S-EPMC7946065 | biostudies-literature
ProteomicsAurora B kinase disrupts the extended conformation of lattice-bound Kinesin-13 MCAK
We reveal that MCAK has a compact conformation in solution using cross-linking and electron microscopy. When MCAK is bound to the microtubule ends, it adopts an extended conformation with the N terminus and neck region of MCAK interacting with the microtubule. Also Aurora Bphosphorylation does not a...
ORGANISM(S): Homo sapiens (Human) 
2019-01-07 | PXD008215 | Pride
Mcak Phosphorylation Microtubules Aurora b 
ProteomicsCross-linking mass spectrometry uncovers protein interactions and functional assemblies in synaptic vesicle membranes
Synaptic vesicles are storage organelles for neurotransmitters in the pre-synaptic cytosol. When an action potential arrives at the pre-synaptic terminal, they fuse with the pre-synaptic membrane and neurotransmitters are released. In this project, we set out to study the protein interactions formed...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-12-09 | PXD020859 | Pride
Chemical cross-linking Mass spectrometry Protein networks Synaptic vesicles Synaptophysin Synaptobrevin-2 
UnknownLRP1 is a neuronal receptor for α-synuclein uptake and spread.
Not available
S-EPMC9438229 | biostudies-literature
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