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TranscriptomicsLocalization profiling of mRNAs associated with microtubules and microtubule bound polysomes in human HeLa cells and unfertilized Xenopus eggs demonstrates conserved localization of mRNAs to mitotic microtubules
Microtubules were purified from mitotic Xenopus or Hela extracts and associated mRNAs were compared to total extract.
ORGANISM(S): Homo sapiens 
2007-12-31 | E-MEXP-1320 | biostudies-arrayexpress
ProteomicsMS-based proteomic characterization of assembled microtubules
Microtubules are dynamic polymers that interconvert between phases of growth and shrinkage, yet they somehow provide lasting structural stability to cells. Growth involves hydrolysis of GTP-tubulin to GDP-tubulin, which releases energy that is stored within the microtubule lattice and destabilizes i...
ORGANISM(S): Bos taurus (Bovine) 
2024-04-30 | PXD049371 | Pride
Microtubules Tubulin Purification In-vitro assembly Nanolc-ms/ms 
ProteomicsDetermination of the tubulin composition of microtubules
Determination of the tubulin composition of microtubules polymerized from two tubulin hydrolysis-deficient mutants
ORGANISM(S): Homo sapiens (Human) 
2025-01-09 | PXD058083 | Pride
Microtubules Tubulin Gtp cap 
GenomicsPolyglutamylation of microtubules drives neuronal remodeling
Polyglutamylation of microtubules drives neuronal remodeling
PRJNA1261042 | ENA
TranscriptomicsMonopolar attachment of sister kinetochores at meiosis I requires casein kinase 1.
The segregation of maternal centromeres away from the paternal ones during the first division of meiosis depends on the attachment of sister kinetochores to microtubules emanating from the same spindle pole. In budding yeast monopolar attachment requires the recruitment to kinetochores of a protein ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-10 | E-GEOD-4792 | biostudies-arrayexpress
GenomicsNucleus deformation by microtubules during early steps of hematopoiesis
Nucleus deformation by microtubules during early steps of hematopoiesis
PRJNA661035 | ENA
GenomicsSystematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi
Various small RNA libraries from purified microtubules or Xiwi immunoprecipitates or total extract from X.tropicalis or X.laevis egg extract. Various small RNA libraries from single X.tropicalis eggs. Analysis of this series of files is described in the manuscript "Systematic and single cell ...
ORGANISM(S): Xenopus (Silurana) tropicalis 
2009-08-31 | E-GEOD-15556 | biostudies-arrayexpress
ProteomicsThe HAUS6 CH domain binds microtubules to orient augmin for microtubule branching
Branching microtubule nucleation is a key mechanism for mitotic and meiotic spindle assembly and requires the hetero-octameric augmin complex. Augmin recruits the major microtubule nucleator, the γ-tubulin ring complex, to pre-existing microtubules to direct the formation of new microtubules in a de...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Homo sapiens (Human) Escherichia coli Drosophila melanogaster (Fruit fly) Xenopus laevis (African clawed frog) 
2025-07-30 | PXD060099 | Pride
Microtubule nucleation Microtubules Cell division 
ProteomicsAurora B kinase disrupts the extended conformation of lattice-bound Kinesin-13 MCAK
We reveal that MCAK has a compact conformation in solution using cross-linking and electron microscopy. When MCAK is bound to the microtubule ends, it adopts an extended conformation with the N terminus and neck region of MCAK interacting with the microtubule. Also Aurora Bphosphorylation does not a...
ORGANISM(S): Homo sapiens (Human) 
2019-01-07 | PXD008215 | Pride
Mcak Phosphorylation Microtubules Aurora b 
ProteomicsA systematic screening pipeline identifies a new regulator of tyrosinated microtubule dynamics
Tubulin detyrosination is a reversible post-translational modification thought to be important for processes including the generation of cell polarity and cell division. The Y/ΔY cycle does not affect the intrinsic properties of microtubules per se, but rather influences the cohort of microtubule-as...
ORGANISM(S): Homo sapiens (Human) 
2022-08-16 | PXD030448 | Pride
Microtubule Detyrosination Tmt6 Human Tyrosination Lc-ms/ms 
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