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ProteomicsAntibody-free targeted proteomics assay for absolute measurement of α-tubulin acetylation
Acetylation of α-tubulin at conserved lysine 40 (K40) amino acid residue regulates microtubule dynamics and controls a wide range of cellular activities. Dysregulated microtubule dynamics characterised by differential α-tubulin acetylation is a hallmark of cancer, neurodegeneration and other complex...
ORGANISM(S): Homo Sapiens Mus Musculus 
2020-07-30 | PXD018626 | panorama
Proteomicsβ-tubulin phosphorylation by Chk1 is required for normal spindle formation during cell division
The mitotic spindle is a microtubule-based apparatus that is responsible for accurate segregation of chromosomes into two daughter cells. In this study, we show that the DNA damage kinase Chk1 is required for optimal density and efficient polymerization of spindle microtubules during unperturbed mit...
ORGANISM(S): Homo sapiens (Human) Spodoptera frugiperda 
2026-06-08 | PXD074543 | Pride
Atr Atrip Topbp1 Centrosome Chromosome segregation -tubulin Chk1 Cell proliferation Mitotic spindle 
ProteomicsX-link of KIF2C-tubulin complex with EDC
KIF2-tubulin complex was crosslinked with EDC, trypsinized, and analyzed by LC-MS.
ORGANISM(S): Sus Scrofa Mus Musculus (mouse) 
2017-09-13 | PXD007152 | JPOST Repository
ProteomicsX-link of KIF2C-tubulin complex with DSSO
KIF2-tubulin complex was crosslinked with DSSO, trypsinized, and analyzed by LC-MS.
ORGANISM(S): Sus Scrofa Mus Musculus (mouse) 
2017-09-13 | PXD007142 | JPOST Repository
ProteomicsDetermination of the tubulin composition of microtubules
Determination of the tubulin composition of microtubules polymerized from two tubulin hydrolysis-deficient mutants
ORGANISM(S): Homo sapiens (Human) 
2025-01-09 | PXD058083 | Pride
Microtubules Tubulin Gtp cap 
ProteomicsCross-linking of the microtubule binding N-terminal domain of the companion of cellulose synthase 1 protein (CC1∆C223) from Arabidopsis thaliana with tubulin
In this study, we use Cross-linking Mass Spectrometry to identify the interaction sites of the microtubule binding N-terminal domain of the companion of cellulose synthase 1 protein (CC1∆C223) from Arabidopsis thaliana with tubulin. We consistently detected four cross-linked peptides of CC1∆C223 to ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-01-08 | PXD009260 | Pride
Arabidopsis thaliana Cx-ms Cross-linking Microtubule Tubulin 
ProteomicsMetabolic regulation of cytoskeleton functions by HDAC6-catalyzed α-tubulin lactylation
Metabolic regulation of cytoskeleton functions by HDAC6-catalyzed α-tubulin lactylation
ORGANISM(S): Mus Musculus 
PXD055797 |
ProteomicsAutoregulation of tubulin mRNA via reversible TTC5 sequestration by soluble ab-tubulins
Microtubule cytoskeleton, built from heterodimers of α and β-tubulins, is critically important to physically organize cells, mediate intracellular transport and power cell division. The availability of soluble αβ-tubulins influences the biomechanical properties and functions of microtubules. When pr...
ORGANISM(S): Homo sapiens (Human) 
2024-10-18 | PXD050483 | Pride
Microtubules Ttc5 Hdx-ms Mrna Tubulin 
ProteomicsElongator is a microtubule polymerase selective for poly-glutamylated tubulin: ELP456 to Tubulin crosslinking
Elongator is a tRNA-modifying complex regulating protein translation. Recently, a moonlighting function of Elongator has been identified in regulating the polarization of the microtubule cytoskeleton during asymmetric cell division. Elongator induces symmetry breaking of the anaphase midzone by sele...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2025-05-07 | PXD058480 | Pride
Tubulin crosslinking elp456 
ProteomicsParthenolide-treated HeLa cells and purified tubulin
Purified human tubulin treated with parthenolide and HeLa cells treated in vivo with parthenolide
ORGANISM(S): Homo sapiens (Human) 
2021-01-22 | PXD020113 | Pride
Parthenolide Q exactive Tubulin Homo sapiens 
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